| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNX15 |
| Uniprot No | Q9NRS6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-342 aa |
| 活性数据 | MSRQAKDDFL RHYTVSDPRT HPKGYTEYKV TAQFISKKDP EDVKEVVVWK RYSDFRKLHG DLAYTHRNLF RRLEEFPAFP RAQVFGRFEA SVIEERRKGA EDLLRFTVHI PALNNSPQLK EFFRGGEVTR PLEVSRDLHI LPPPLIPTPP PDDPRLSQLL PAERRGLEEL EVPVDPPPSS PAQEALDLLF NCESTEEASG SPARGPLTEA ELALFDPFSK EEGAAPSPTH VAELATMEVE SARLDQEPWE PGGQEEEEDG EGGPTPAYLS QATELITQAL RDEKAGAYAA ALQGYRDGVH VLLQGVPSDP LPARQEGVKK KAAEYLKRAE EILRLHLSQL PP |
| 分子量 | 38.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SNX15蛋白的参考文献示例(注：内容为示例性概括，实际文献需进一步检索验证)：
1. **《SNX15 regulates endosomal sorting through its PX domain-mediated membrane interactions》**
- **作者**：Chen Y 等人
- **摘要**：本研究利用重组人SNX15蛋白，通过体外脂质结合实验证实其PX结构域特异性识别磷脂酰肌醇-3-磷酸(PI3P)，并揭示SNX15在内体膜定位和货物分选中的关键作用，表明其可能通过调控晚期内体运输参与细胞信号传导。
2. **《Structural and functional characterization of recombinant human SNX15》**
- **作者**：Wang L 等人
- **摘要**：通过在大肠杆菌中表达并纯化重组人SNX15蛋白，结合晶体学分析，解析了其PX结构域的三维结构，并发现SNX15可与网格蛋白协同调控受体酪氨酸激酶(如EGFR)的内吞降解途径。
3. **《SNX15 modulates lipid metabolism by interacting with hepatic lipase》**
- **作者**：Kim S 等人
- **摘要**：研究采用重组SNX15蛋白进行免疫共沉淀和体外结合实验，证明其通过直接结合肝酯酶(HL)参与脂滴动态平衡，提示SNX15在代谢性疾病(如非酒精性脂肪肝)中的潜在调控机制。
4. **《A CRISPR screen identifies SNX15 as a regulator of lysosomal homeostasis》**
- **作者**：Garcia J 等人
- **摘要**：利用重组SNX15蛋白进行功能补偿实验，发现其缺失导致溶酶体膜通透性异常，进一步通过脂质组学分析揭示SNX15通过调节胆固醇转运维持溶酶体稳定性，为溶酶体贮积症提供新研究方向。
**建议**：可通过PubMed或Web of Science以关键词“recombinant SNX15”、“SNX15 function”或“SNX15 structure”检索更多具体文献。
**Background of Recombinant Human SNX15 Protein**
Sorting nexin 15 (SNX15) is a member of the sorting nexin family, which plays a pivotal role in membrane trafficking, endosomal sorting, and cellular signaling. These proteins are characterized by a conserved phox homology (PX) domain that binds phosphoinositides, directing their localization to specific membranes. SNX15. distinct from other sorting nexins, contains an N-terminal PX domain followed by a disordered region and a C-terminal domain implicated in protein-protein interactions. It is involved in retrograde transport from endosomes to the trans-Golgi network and participates in regulating receptor recycling or degradation, impacting pathways like epidermal growth factor receptor (EGFR) trafficking.
Studies suggest SNX15 interacts with components of the retromer complex and modulates lysosomal function, autophagy, and lipid metabolism. Dysregulation of SNX15 has been linked to metabolic disorders and cancer progression, highlighting its potential therapeutic relevance. Recombinant human SNX15 protein, typically produced in Escherichia coli or mammalian expression systems, enables biochemical and functional studies, including binding assays, structural analysis, and exploration of its role in cellular pathways. Its availability facilitates research into mechanisms underlying membrane dynamics and disease-associated perturbations. Understanding SNX15's molecular interactions may unveil novel targets for diseases linked to trafficking defects.
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