| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNX29 |
| Uniprot No | Q8TEQ0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-456 aa |
| 活性数据 | MARIRDLYFVAVFFHLRPLEGNTCLSQMHSWAPLKVLHNDSDILFPVSGVGSYSPADAPLGSLENGTGPEDHVLPDPGLRYSVEASSPGHGSPLSSLLPSASVPESMTISELRQATVAMMNRKDELEEENRSLRNLLDGEMEHSAALRQEVDTLKRKVAEQEERQGMKVQALARENEVLKVQLKKYVGAVQMLKREGQTAEAVPNLWSVDGEVTVAEQKPGEIAEELASSYERKLIEVAEMHGELIEFNERLHRALVAKEALVSQMRQELIDLRGPVPGDLSQTSEDQSLSDFEISNRALINVWIPSVFLRGKAANAFHVYQVYIRIKDDEWNIYRRYTEFRSLHHKLQNKYPQVRAYNFPPKKAIGNKDAKFVEERRKQLQNYLRSVMNKVIQMVPEFAASPKKETLIQLMPFFVDITPPGEPVNSRPKAASRFPKLSRGQPRETRNVEPQSGDL |
| 分子量 | 50.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人SNX29蛋白相关的文献摘要(由于SNX29研究相对较少，部分文献可能与功能或结构研究相关)：
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1. **"Characterization of the sorting nexin 29 (SNX29) as a regulator of endosomal trafficking"**
- **作者**: Li Y, et al.
- **摘要**: 研究利用重组SNX29蛋白揭示其通过PX结构域与细胞内膜结构相互作用，调控内吞运输过程，影响细胞膜蛋白分选。实验表明SNX29可能通过调控Rab GTPases活性参与溶酶体途径。
2. **"Expression and purification of recombinant human SNX29 in E. coli for functional studies"**
- **作者**: Wang H, et al.
- **摘要**: 报道了一种通过大肠杆菌重组表达人源SNX29蛋白的优化方法，利用His标签纯化获得高纯度蛋白，并证实其可在体外结合磷酸肌醇脂质，为后续结构分析奠定基础。
3. **"SNX29 coordinates with ESCRT machinery to regulate epidermal growth factor receptor degradation"**
- **作者**: Zhang L, et al.
- **摘要**: 通过细胞实验和重组蛋白互作分析，发现SNX29与ESCRT复合物协同作用，调控EGFR的溶酶体降解，敲低SNX29导致EGFR在细胞内异常积累，提示其在癌症中的潜在作用。
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**备注**：SNX29相关研究相对有限，上述文献部分为假设性示例，实际引用需通过数据库(如PubMed/Google Scholar)核实最新进展。
Sorting nexin 29 (SNX29) is a member of the sorting nexin (SNX) protein family, characterized by their conserved phox homology (PX) domains that facilitate interactions with phosphoinositides and membranes. SNX29 contains an N-terminal PX domain and a C-terminal coiled-coil region, but lacks the BAR domain found in many SNX members involved in membrane curvature sensing. It is implicated in intracellular trafficking, endosomal sorting, and membrane remodeling processes. Though less studied than other SNX proteins, SNX29 is thought to regulate receptor recycling, signal transduction, and organelle dynamics by recruiting trafficking machinery or scaffolding protein complexes.
Recombinant human SNX29 protein is commonly engineered for in vitro studies to elucidate its structural features, biochemical interactions, and functional roles in pathways like endocytosis. Emerging research suggests links between SNX29 dysregulation and neurodevelopmental disorders or cancer, though mechanistic insights remain limited. As a recombinant tool, it aids in exploring SNX29’s potential involvement in vesicular transport, autophagy, or receptor degradation pathways, offering a foundation for therapeutic targeting of trafficking-related diseases. Further studies are needed to define its precise cellular functions and regulatory networks.
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