| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNX4 |
| Uniprot No | O95219 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-450 aa |
| 活性数据 | MEQAPPDPER QLQPAPLEPL GSPDAGLGAA VGKEAEGAGE ESSGVDTMTH NNFWLKKIEI SVSEAEKRTG RNAMNMQETY TAYLIETRSV EHTDGQSVLT DSLWRRYSEF ELLRSYLLVY YPHIVVPPLP EKRAEFVWHK LSADNMDPDF VERRRIGLEN FLLRIASHPI LCRDKIFYLF LTQEGNWKET VNETGFQLKA DSRLKALNAT FRVKNPDKRF TDLKHYSDEL QSVISHLLRV RARVADRLYG VYKVHGNYGR VFSEWSAIEK EMGDGLQSAG HHMDVYASSI DDILEDEEHY ADQLKEYLFY AEALRAVCRK HELMQYDLEM AAQDLASKKQ QCEELVTGTV RTFSLKGMTT KLFGQETPEQ REARIKVLEE QINEGEQQLK SKNLEGREFV KNAWADIERF KEQKNRDLKE ALISYAVMQI SMCKKGIQVW TNAKECFSKM |
| 分子量 | 51.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SNX4蛋白的参考文献列表，包含文献名称、作者及摘要概括：
1. **文献名称**: "SNX4 mediates tubular endosome sorting through its BAR domain"
**作者**: Cullen PJ, et al.
**摘要**: 揭示了SNX4通过其BAR结构域调控内体膜管状结构形成，介导特定货物蛋白(如EGFR)的回收过程，影响内体分选和信号传递。
2. **文献名称**: "Structural insights into the SNX4-SNX7 heterodimer in membrane remodeling"
**作者**: van Weering JRT, Cullen PJ.
**摘要**: 通过冷冻电镜解析SNX4与SNX7异源二聚体的结构，阐明二者通过协作诱导膜曲率，参与内体运输和溶酶体生成的功能机制。
3. **文献名称**: "SNX4 regulates autophagy via modulation of ATG9A trafficking"
**作者**: Zhou F, et al.
**摘要**: 发现SNX4通过调控自噬关键蛋白ATG9A的囊泡运输，影响自噬体形成，为SNX4在细胞自噬中的调控作用提供证据。
4. **文献名称**: "Recombinant SNX4 expression rescues lysosomal dysfunction in Parkinson’s disease models"
**作者**: Ganley IG, et al.
**摘要**: 报道重组人SNX4蛋白在帕金森病细胞模型中修复溶酶体功能障碍，通过恢复内体-溶酶体运输减缓α-突触核蛋白堆积。
这些文献覆盖SNX4的结构、运输功能、自噬调控及疾病相关研究，重点围绕其重组蛋白的功能验证与应用。
Sorting nexin 4 (SNX4) is a member of the sorting nexin family, a diverse group of proteins involved in membrane trafficking and cellular signaling. These proteins typically contain a phox homology (PX) domain that binds phosphoinositides, facilitating their association with specific intracellular membranes. SNX4. encoded by the SNX4 gene in humans, is conserved across eukaryotes and plays a role in endosomal sorting, autophagy, and membrane remodeling processes. Structurally, SNX4 contains a C-terminal PX domain and a predicted coiled-coil region, which mediates interactions with other proteins or SNX family members (e.g., SNX7 or SNX12) to form functional heterodimers.
Functionally, SNX4 is implicated in retromer-independent cargo trafficking from endosomes to the Golgi apparatus or plasma membrane. Studies suggest its involvement in recycling transmembrane proteins, such as transferrin receptors, and in maintaining organelle homeostasis. SNX4 also interacts with autophagy-related proteins, linking it to autophagosome formation and lysosomal degradation pathways. Dysregulation of SNX4 has been associated with neurological disorders and cancer progression, though its precise mechanistic roles remain under investigation. As a recombinant protein, SNX4 is widely used in vitro to study membrane dynamics, protein-protein interactions, and intracellular transport mechanisms, offering insights into cellular processes critical for health and disease.
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