| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNX27 |
| Uniprot No | Q96L92 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-541 aa |
| 活性数据 | MADEDGEGIH PSAPHRNGGG GGGGGSGLHC AGNGGGGGGG PRVVRIVKSE SGYGFNVRGQ VSEGGQLRSI NGELYAPLQH VSAVLPGGAA DRAGVRKGDR ILEVNHVNVE GATHKQVVDL IRAGEKELIL TVLSVPPHEA DNLDPSDDSL GQSFYDYTEK QAVPISVPRY KHVEQNGEKF VVYNVYMAGR QLCSKRYREF AILHQNLKRE FANFTFPRLP GKWPFSLSEQ QLDARRRGLE EYLEKVCSIR VIGESDIMQE FLSESDENYN GVSDVELRVA LPDGTTVTVR VKKNSTTDQV YQAIAAKVGM DSTTVNYFAL FEVISHSFVR KLAPNEFPHK LYIQNYTSAV PGTCLTIRKW LFTTEEEILL NDNDLAVTYF FHQAVDDVKK GYIKAEEKSY QLQKLYEQRK MVMYLNMLRT CEGYNEIIFP HCACDSRRKG HVITAISITH FKLHACTEEG QLENQVIAFE WDEMQRWDTD EEGMAFCFEY ARGEKKPRWV KIFTPYFNYM HECFERVFCE LKWRKENIFQ MARSQQRDVA T |
| 分子量 | 61.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SNX27蛋白的3篇关键文献参考，简明列举如下：
1. **文献名称**：*SNX27 mediates retromer cargo entry by sorting large domains via a highly flexible interface*
**作者**：Ghosh, P., et al.
**摘要**：该研究解析了SNX27蛋白与不同受体的结合机制，揭示其通过PDZ结构域识别特定信号肽介导细胞内运输，并参与溶酶体途径与回收途径的货物分选(Nature Communications, 2020)。
2. **文献名称**：*SNX27 links PDZ domain–dependent cargo recognition with retromer assembly in endosomal transport*
**作者**：Temkin, P., et al.
**摘要**：本文阐述了SNX27通过PDZ结构域结合含特定基序的跨膜蛋白(如β2肾上腺素受体)，并与逆向运输复合体(retromer)协作调控细胞表面受体循环，影响神经元功能(Cell, 2011)。
3. **文献名称**：*Loss of SNX27 causes impaired synaptic function and cognitive deficits in down syndrome*
**作者**：Wang, X., et al.
**摘要**：研究指出SNX27缺失导致唐氏综合征模型小鼠中谷氨酸受体错误分选至溶酶体降解，破坏突触可塑性，提示SNX27在神经发育疾病中的关键作用(Nature Medicine, 2013)。
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**附注**：以上文献均为SNX27功能研究的经典论文，重点关注其结构机制与疾病关联。如需补充具体期刊卷期或DOI，请进一步说明。
**Background of Recombinant Human SNX27 Protein**
SNX27 (Sorting Nexin 27) is a cytoplasmic protein belonging to the sorting nexin family, which plays critical roles in membrane trafficking, endosomal sorting, and signal transduction. It is distinguished by a unique N-terminal PDZ domain that enables interactions with cargo proteins containing PDZ-binding motifs, facilitating their recycling from endosomes to the plasma membrane. This function is essential for maintaining cellular homeostasis, particularly in neurons, where SNX27 regulates the surface expression of receptors (e.g., GPCRs, ion channels) and transporters implicated in synaptic plasticity and neurotransmission.
SNX27 collaborates with the retromer complex, a conserved trafficking machinery, to sort cargo away from lysosomal degradation. Dysregulation of SNX27 is linked to neurodevelopmental and neurodegenerative disorders. Notably, SNX27 deficiency has been associated with intellectual disabilities and Down syndrome, while its interaction with β-amyloid precursor protein (APP) highlights potential roles in Alzheimer’s disease pathogenesis.
Recombinant human SNX27 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), retains functional domains required for studying its structure, binding partners, and trafficking mechanisms. It serves as a vital tool for dissecting endosomal sorting pathways, screening therapeutic compounds, and modeling diseases linked to SNX27 dysfunction. Its applications span neuroscience, cell biology, and drug discovery, underscoring its relevance in understanding and treating trafficking-related pathologies.
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