| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SOAT2 |
| Uniprot No | O75908 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-522 aa |
| 活性数据 | MEPGGARLRLQRTEGLGGERERQPCGDGNTETHRAPDLVQWTRHMEAVKAQLLEQAQGQLRELLDRAMREAIQSYPSQDKPLPPPPPGSLSRTQEPSLGKQKVFIIRKSLLDELMEVQHFRTIYHMFIAGLCVFIISTLAIDFIDEGRLLLEFDLLIFSFGQLPLALVTWVPMFLSTLLAPYQALRLWARGTWTQATGLGCALLAAHAVVLCALPVHVAVEHQLPPASRCVLVFEQVRFLMKSYSFLREAVPGILRARRGEGIQAPSFSSYLYFLFCPTLIYRETYPRTPYVRWNYVAKNFAQALGCVLYACFILGRLCVPVFANMSREPFSTRALVLSILHATLPGIFMLLLIFFAFLHCWLNAFAEMLRFGDRMFYRDWWNSTSFSNYYRTWNVVVHDWLYSYVYQDGLRLLGARARGVAMLGVFLVSAVAHEYIFCFVLGFFYPVMLILFLVIGGMLNFMMHDQRTGPAWNVLMWTMLFLGQGIQVSLYCQEWYARRHCPLPQATFWGLVTPRSWSCHT |
| 分子量 | 86.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条与重组人SOAT2(甾醇O-酰基转移酶2)蛋白相关的文献摘要概括：
1. **《Functional expression and characterization of human sterol O-acyltransferase 2 (SOAT2) in insect cells》**
- **作者**：Chang, T.Y. et al.
- **摘要**：该研究利用杆状病毒-昆虫细胞系统成功表达重组人SOAT2蛋白，验证其在胆固醇酯合成中的催化活性，并通过突变分析揭示了关键功能结构域。
2. **《Role of SOAT2 in hepatic lipid metabolism: Insights from recombinant protein studies》**
- **作者**：Lee, R.G. & Rudel, L.L.
- **摘要**：通过在大肠杆菌中重组表达人SOAT2.研究其与肝脏胆固醇酯代谢的关联，发现其活性受脂肪酸底物浓度调控，并与动脉粥样硬化风险相关。
3. **《Purification and biochemical characterization of recombinant human SOAT2 for drug screening》**
- **作者**：Zhang, X. et al.
- **摘要**：报道了一种高效纯化重组人SOAT2蛋白的方法(哺乳动物细胞表达系统)，并基于此开发了针对胆固醇酯化抑制剂的体外高通量筛选平台。
(注：以上文献为示例性内容，实际引用时需结合具体文章核实。)
Sterol O-acyltransferase 2 (SOAT2), also known as acyl-coenzyme A:cholesterol acyltransferase 2 (ACAT2), is a key enzyme in intracellular cholesterol metabolism. Predominantly expressed in hepatocytes and intestinal enterocytes, SOAT2 catalyzes the esterification of free cholesterol into cholesteryl esters, a critical step in lipid storage and lipoprotein assembly. This enzymatic activity enables the incorporation of cholesteryl esters into nascent very-low-density lipoproteins (VLDL) in the liver and chylomicrons in the intestine, facilitating systemic cholesterol transport.
SOAT2 has gained significant research attention due to its role in modulating plasma cholesterol levels and potential link to atherosclerosis. Unlike its isoform SOAT1 (ubiquitously expressed for cytoplasmic lipid droplet formation), SOAT2 specifically contributes to lipoprotein-associated cholesterol secretion. Animal studies suggest that SOAT2 inhibition reduces atherogenic lipoprotein production without causing tissue cholesterol accumulation, making it an attractive therapeutic target for cardiovascular diseases.
Recombinant human SOAT2 protein, typically produced in mammalian expression systems, retains catalytic activity and structural integrity for functional studies. It serves as a vital tool for elucidating cholesterol esterification mechanisms, screening pharmacological inhibitors, and investigating genetic variants. Current research focuses on developing tissue-specific SOAT2 modulators to balance cholesterol homeostasis while minimizing off-target effects.
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