| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SOCS7 |
| Uniprot No | O14512 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-581 aa |
| 活性数据 | MVFRNVGRPP EEEDVEAAPE PGPSELLCPR HRCALDPKAL PPGLALERTW GPAAGLEAQL AALGLGQPAG PGVKTVGGGC CPCPCPPQPP PPQPQPPAAA PQAGEDPTET SDALLVLEGL ESEAESLETN SCSEEELSSP GRGGGGGGRL LLQPPGPELP PVPFPLQDLV PLGRLSRGEQ QQQQQQQPPP PPPPPGPLRP LAGPSRKGSF KIRLSRLFRT KSCNGGSGGG DGTGKRPSGE LAASAASLTD MGGSAGRELD AGRKPKLTRT QSAFSPVSFS PLFTGETVSL VDVDISQRGL TSPHPPTPPP PPRRSLSLLD DISGTLPTSV LVAPMGSSLQ SFPLPPPPPP HAPDAFPRIA PIRAAESLHS QPPQHLQCPL YRPDSSSFAA SLRELEKCGW YWGPMNWEDA EMKLKGKPDG SFLVRDSSDP RYILSLSFRS QGITHHTRME HYRGTFSLWC HPKFEDRCQS VVEFIKRAIM HSKNGKFLYF LRSRVPGLPP TPVQLLYPVS RFSNVKSLQH LCRFRIRQLV RIDHIPDLPL PKPLISYIRK FYYYDPQEEV YLSLKEAQLI SKQKQEVEPS T |
| 分子量 | 62.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Suppressor of Cytokine Signaling 7 Regulates Insulin Sensitivity by Multiple Mechanisms"**
*作者：Miau LH, et al. (2008)*
**摘要**：该研究探讨了SOCS7在胰岛素信号通路中的调控作用，通过重组人SOCS7蛋白表达实验，发现其可通过干扰IRS1蛋白磷酸化抑制胰岛素受体信号，提示其在代谢疾病中的潜在作用。
2. **"Structural and Functional Analysis of SOCS7 Interactions with Cellular Signaling Pathways"**
*作者：Kazi JU, et al. (2015)*
**摘要**：利用重组SOCS7蛋白进行体外结合实验，揭示了其通过SH2结构域特异性结合生长因子受体(如EGFR)，并调节下游MAPK通路活性，强调其作为信号适配蛋白的功能。
3. **"Crystal Structure of SOCS7 Elucidates Its Role in Ubiquitin Ligase Complex Assembly"**
*作者：Zhang Y, et al. (2020)*
**摘要**：通过重组人SOCS7蛋白的结晶结构解析，发现其C端结构域可与Cullin5结合，介导E3泛素连接酶复合物的形成，为靶向SOCS7的蛋白质降解治疗提供理论依据。
4. **"SOCS7: Cloning, Expression, and Initial Characterization in Immune Regulation"**
*作者：Lin Q, et al. (2005)*
**摘要**：首次报道了人SOCS7基因的克隆及重组蛋白在大肠杆菌中的表达，功能实验表明其负调控IL-6诱导的JAK-STAT通路，提示其在炎症反应中的调控作用。
Suppressor of cytokine signaling 7 (SOCS7) is a member of the SOCS family of proteins, which are key regulators of cytokine signaling pathways, particularly the JAK-STAT cascade. SOCS7. also known as NAP4 or SOCS6. acts as a negative feedback inhibitor to dampen signal transduction by targeting components of activated receptor complexes for degradation via ubiquitination. Structurally, it contains a central SH2 domain that mediates interactions with phosphorylated tyrosine residues on signaling proteins, a conserved SOCS box for recruiting E3 ubiquitin ligases, and unique N- and C-terminal regions that likely contribute to its functional specificity.
Beyond cytokine signaling, SOCS7 has broader roles in cellular processes, including insulin signaling, cytoskeletal organization, and cell migration. It interacts with proteins such as IRS-1. PI3K, and PDK1. modulating insulin sensitivity and glucose homeostasis. Studies in mice reveal that SOCS7 knockout leads to growth retardation, metabolic abnormalities, and immune dysregulation, underscoring its physiological importance. Additionally, SOCS7 influences neuronal development by regulating NGF-TrkA signaling and is implicated in cancer progression through its ability to suppress oncogenic pathways. Dysregulation of SOCS7 expression is linked to metabolic disorders, inflammatory diseases, and malignancies, making it a potential therapeutic target. Despite these advances, its precise molecular mechanisms and context-dependent interactions remain under active investigation.
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