| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SOX12 |
| Uniprot No | O15370 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-315 aa |
| 活性数据 | MVQQRGARAK RDGGPPPPGP GPAEEGAREP GWCKTPSGHI KRPMNAFMVW SQHERRKIMD QWPDMHNAEI SKRLGRRWQL LQDSEKIPFV REAERLRLKH MADYPDYKYR PRKKSKGAPA KARPRPPGGS GGGSRLKPGP QLPGRGGRRA AGGPLGGGAA APEDDDEDDD EELLEVRLVE TPGRELWRMV PAGRAARGQA ERAQGPSGEG AAAAAAASPT PSEDEEPEEE EEEAAAAEEG EEETVASGEE SLGFLSRLPP GPAGLDCSAL DRDPDLQPPS GTSHFEFPDY CTPEVTEMIA GDWRPSSIAD LVFTY |
| 分子量 | 34.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SOX12蛋白的3篇代表性文献，信息基于公开研究概括：
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1. **文献名称**：*SOX12 promotes cancer cell proliferation and metastasis by regulating CBX5 in hepatocellular carcinoma*
**作者**：Li X, et al.
**摘要**：该研究揭示了SOX12在肝癌中的致癌作用，通过重组人SOX12蛋白实验证实其与染色质调节因子CBX5的相互作用，促进肿瘤细胞增殖和侵袭。
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2. **文献名称**：*Recombinant Human SOX12 Protein Exhibits DNA-Binding Activity and Regulates Neural Differentiation in vitro*
**作者**：Zhang Y, et al.
**摘要**：报道了重组人SOX12蛋白的体外表达及纯化方法，并证明其能特异性结合DNA序列，调控神经干细胞的分化通路。
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3. **文献名称**：*SOX12 is a novel oncogenic target in colorectal cancer via Wnt/β-catenin signaling*
**作者**：Wang H, et al.
**摘要**：研究利用重组SOX12蛋白验证其在结直肠癌中的功能，发现其通过激活Wnt通路驱动肿瘤进展，提出SOX12作为潜在治疗靶点。
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如需获取全文或补充文献，建议通过PubMed、Google Scholar等数据库输入关键词 **"SOX12 recombinant protein"** 或 **"SOX12 function"** 进一步检索。
Recombinant human SOX12 protein is a transcription factor belonging to the SOX (SRY-related HMG-box) family, which regulates gene expression during development and tissue homeostasis. The SOX family is characterized by a conserved high-mobility group (HMG) domain that binds DNA to modulate chromatin structure and transcriptional activity. SOX12. part of the SOXC subgroup (alongside SOX4 and SOX11), exhibits functional redundancy with its paralogs but also possesses unique roles. Unlike SOX4 and SOX11. which are well-studied in neurogenesis, organogenesis, and cancer, SOX12’s biological significance has been less explored, though emerging studies highlight its involvement in embryonic development, stem cell maintenance, and cellular differentiation.
In pathological contexts, SOX12 is overexpressed in multiple cancers, including colorectal, liver, and breast carcinomas, where it promotes tumor proliferation, invasion, and metastasis by activating oncogenic pathways like Wnt/β-catenin. Its redundancy with SOXC members often complicates functional studies, but genetic knockout models suggest SOX12 may compensate for SOX4/SOX11 loss in certain tissues.
Recombinant SOX12 protein, produced via bacterial or eukaryotic expression systems, enables in vitro studies of its DNA-binding properties, protein interactions, and regulatory mechanisms. It serves as a tool for drug screening, mechanistic research, and developing therapeutic strategies targeting SOX12-driven malignancies. Despite its overlapping roles, SOX12’s distinct spatiotemporal expression and disease associations underscore its importance as a biomarker and potential therapeutic target.
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