| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SOX8 |
| Uniprot No | P57073 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-446 aa |
| 活性数据 | MLDMSEARSQPPCSPSGTASSMSHVEDSDSDAPPSPAGSEGLGRAGVAVGGARGDPAEAA DERFPACIRDAVSQVLKGYDWSLVPMPVRGGGGGALKAKPHVKRPMNAFMVWAQAARRKL ADQYPHLHNAELSKTLGKLWRLLSESEKRPFVEEAERLRVQHKKDHPDYKYQPRRRKSAK AGHSDSDSGAELGPHPGGGAVYKAEAGLGDGHHHGDHTGQTHGPPTPPTTPKTELQQAGA KPELKLEGRRPVDSGRQNIDFSNVDISELSSEVMGTMDAFDVHEFDQYLPLGGPAPPEPG QAYGGAYFHAGASPVWAHKSAPSASASPTETGPPRPHIKTEQPSPGHYGDQPRGSPDYGS CSGQSSATPAAPAGPFAGSQGDYGDLQASSYYGAYPGYAPGLYQYPCFHSPRRPYASPLL NGLALPPAHSPTSHWDQPVYTTLTRP |
| 分子量 | 47.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SOX8蛋白的参考文献示例(注：文献为模拟示例，可能不完全对应实际发表内容)：
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1. **《Sox8 regulates neural crest stem cell properties in vitro》**
*作者：Wegner M, Stolt CC*
摘要：该研究利用重组人SOX8蛋白，揭示了其在神经嵴干细胞中的转录调控作用，证明SOX8通过结合特定DNA序列维持干细胞多能性和迁移能力。
2. **《Functional analysis of SOX8 in testicular development using recombinant protein》**
*作者：Sock E, Schmidt K, Bergemann J*
摘要：通过表达和纯化重组人SOX8蛋白，研究者发现其在小鼠睾丸发育中与SOX9协同作用，调控支持细胞分化及激素信号通路。
3. **《Sox8 interacts with β-catenin to promote glioblastoma progression》**
*作者：Liu Y, Zhang J, et al.*
摘要：该研究使用重组SOX8蛋白进行体外结合实验，证明其与β-catenin形成复合物，激活Wnt信号通路，促进胶质母细胞瘤的侵袭和增殖。
4. **《Recombinant SOX8 protein structure and dimerization dynamics》**
*作者：Holland CM, Koopman P*
摘要：通过X射线晶体学分析重组人SOX8蛋白结构，揭示了其二聚化界面及与其他SOX家族成员(如SOX9)的功能差异，为突变相关疾病提供分子基础。
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建议通过PubMed或Google Scholar搜索以下关键词获取真实文献：
**SOX8 recombinant protein expression / SOX8 transcriptional regulation / SOX8 disease mechanism**
研究领域多集中于发育生物学、癌症及生殖系统疾病。
SOX8. a member of the SOX (SRY-related HMG box) transcription factor family, plays critical roles in developmental processes and cellular differentiation. Encoded by the *SOX8* gene located on human chromosome 16. the protein contains a conserved HMG (High Mobility Group) DNA-binding domain, enabling sequence-specific interactions with regulatory regions of target genes. SOX8 exhibits overlapping functions with SOX9 and SOX10. particularly in neural crest development, neurogenesis, and sex determination. It is expressed in various tissues, including the nervous system, testes, and kidneys, where it regulates gene networks involved in cell fate specification, survival, and differentiation.
In vertebrates, SOX8 contributes to gliogenesis, oligodendrocyte maturation, and maintenance of male sexual differentiation by activating testis-specific pathways. Its role in sex development is partially redundant with SOX9. but loss-of-function studies highlight its unique contributions to Sertoli cell function and fertility. Dysregulation of SOX8 has been implicated in disorders such as cancer (e.g., glioblastoma, melanoma), neurodevelopmental defects, and disorders of sex development (DSD).
Recombinant human SOX8 protein is typically produced in *E. coli* or mammalian expression systems, often fused with tags (e.g., His, GST) for purification and detection. It serves as a vital tool for studying DNA-protein interactions, transcriptional regulation, and disease mechanisms. Researchers use it in assays like EMSA, chromatin immunoprecipitation (ChIP), and *in vitro* differentiation models to dissect its biological roles and therapeutic potential.
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