| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SP2 |
| Uniprot No | Q02086 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-249 aa |
| 活性数据 | MAATAAVSPSDYLQPAASTTQDSQPSPLALLAATCSKIGPPAVEAAVTPPAPPQPTPRKLVPIKPAPLPLSPGKNSFGILSSKGNILQIQGSQLSASYPGGQLVFAIQNPTMINKGTRSNANIQYQAVPQIQASNSQTIQVQPNLTNQIQIIPGTNQAIITPSPSSHKPVPIKPAPIQKSSTTTTPVQSGANVVKLTGGGGNVTLTLPVNNLVNASDTGAPTQLLTASCQTGMLNSTRMFLFLAFINVL |
| 分子量 | 52 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SP2蛋白的3篇参考文献的简要列举(注：SP2相关研究文献较少，以下为示例性内容，实际文献需根据具体研究方向检索)：
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1. **文献名称**：*SP2 regulates self-renewal of pluripotent stem cells through direct transcriptional activation*
**作者**：Smith J, et al.
**摘要**：研究报道了重组人SP2蛋白在小鼠胚胎干细胞中的功能，证明其通过结合Oct4和Nanog基因启动子区维持多能性，并验证了原核表达的重组SP2在体外的DNA结合活性。
2. **文献名称**：*Production and characterization of recombinant human SP2 protein in Escherichia coli*
**作者**：Li Y, et al.
**摘要**：描述利用大肠杆菌表达系统高效制备重组SP2蛋白，通过His标签纯化和结构鉴定，证实该蛋白具有体外调控特定基因转录的功能，为后续机制研究提供工具。
3. **文献名称**：*SP2 deficiency disrupts neural differentiation via Wnt/β-catenin signaling*
**作者**：Chen R, et al.
**摘要**：通过重组SP2蛋白处理人类诱导多能干细胞，发现SP2通过抑制Wnt通路调控神经分化，提示其在细胞命运决定中的潜在应用价值。
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(注：若需真实文献，建议在PubMed或Web of Science以“SP2 protein recombinant”或“SP2 transcriptional function”为关键词检索，并补充具体应用场景如“干细胞”或“癌症”等。)
**Background of Recombinant Human SP2 Protein**
Recombinant human SP2 (Specificity Protein 2) protein is a genetically engineered version of the transcription factor encoded by the *SP2* gene, belonging to the SP/KLF family of zinc finger DNA-binding proteins. SP2 plays a regulatory role in gene expression by binding to GC-rich promoter regions, influencing cellular processes such as proliferation, differentiation, and apoptosis. Unlike other SP family members (e.g., SP1 or SP3), SP2 exhibits unique DNA-binding specificity and functional diversity, though its precise biological roles remain less characterized.
Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), recombinant SP2 is purified to high homogeneity, enabling structural and functional studies. Its applications span molecular biology research, including elucidating transcriptional mechanisms, identifying target genes, and exploring interactions with signaling pathways. SP2 has been implicated in diseases like cancer, where dysregulation may contribute to tumor progression.
The availability of recombinant SP2 facilitates drug discovery, biomarker studies, and mechanistic insights into its dual roles in homeostasis and pathology. Ongoing research aims to clarify its context-dependent functions and therapeutic potential.
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