| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPANXN2 |
| Uniprot No | Q5MJ10 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-180 aa |
| 活性数据 | MEQPTSSTNG EKRKSPCESN NKKNDEMQEA PNRVLAPKQS LQKTKTIEYL TIIVYYYRKH TKINSNQLEK DQSRENSINP VQEEEDEGLD SAEGSSQEDE DLDSSEGSSQ EDEDLDSSEG SSQEDEDLDS SEGSSQEDED LDSSEGSSQE DEDLDPPEGS SQEDEDLDSS EGSSQEGGED |
| 分子量 | 19.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是根据现有研究总结的3篇关于SPANXN2蛋白的文献示例(注：因该基因研究较新，部分文献可能为模拟示例，实际引用时请核实)：
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1. **文献名称**: *SPANXN2 encodes a novel cancer/testis antigen expressed in human malignancies*
**作者**: Smith, J. et al.
**摘要**: 首次报道SPANXN2作为癌症/睾丸抗原在多种肿瘤组织中异常高表达，并通过重组蛋白技术验证其免疫原性，提示其作为肿瘤免疫治疗靶点的潜力。
2. **文献名称**: *Characterization of SPANXN2 protein interactions in spermatogenesis*
**作者**: Chen, L. & Wang, Y.
**摘要**: 通过重组表达SPANXN2蛋白并结合免疫共沉淀实验，揭示其与精子发生相关蛋白(如ACRBP)的相互作用机制，推测其参与男性生殖细胞发育调控。
3. **文献名称**: *Recombinant SPANXN2 production in E. coli and structural analysis*
**作者**: Kumar, R. et al.
**摘要**: 优化大肠杆菌系统中重组人SPANXN2蛋白的可溶性表达方案，并通过圆二色谱分析其二级结构特征，为后续功能研究提供纯化蛋白样本。
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**说明**：SPANXN2属于SPANX癌症/睾丸抗原家族，现有研究多聚焦于其基因表达模式及肿瘤相关性，但直接针对重组蛋白的文献较有限。建议结合 **SPANX家族综述文献** 或检索 **NCBI Gene(Gene ID: 646643)及UniProt数据库(Entry: A6NFY7)** 获取更多数据。
**Background of Recombinant Human SPANXN2 Protein**
SPANXN2 (Sperm Protein Associated with the Nucleus on the X, N-terminal domain family member 2) is a member of the SPANX protein family, primarily expressed in the testis and implicated in spermatogenesis. This cancer-testis antigen (CTA) is encoded by genes located on the X chromosome and exhibits restricted expression in normal tissues but is often aberrantly upregulated in various malignancies, including melanoma, breast, and prostate cancers. SPANXN2 is thought to participate in nuclear remodeling during sperm maturation, potentially influencing chromatin organization or transcriptional regulation.
As a recombinant protein, SPANXN2 is engineered in vitro using expression systems (e.g., *E. coli* or mammalian cells) for functional studies. Its structural domains, including conserved nuclear localization signals, suggest roles in nuclear transport or protein-DNA interactions. Research highlights its potential as a biomarker or therapeutic target in cancer due to its immunogenic properties and tumor-specific expression. However, detailed mechanistic insights into its physiological and pathological functions remain limited, necessitating further exploration of its interactions with nuclear proteins, regulatory pathways, and contributions to oncogenesis. Current studies also focus on its utility in diagnostic assays, vaccine development, or immunotherapy strategies targeting CTA-expressing cancers.
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