| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPATA17 |
| Uniprot No | Q96L03 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-361 aa |
| 活性数据 | MATLARLQAR SSTVGNQYYF RNSVVDPFRK KENDAAVKIQ SWFRGCQVRA YIRHLNRIVT IIQKWWRSFL GRKQYQLTVQ VAYYTMMMNL YNAMAVRIQR RWRGYRVRKY LFNYYYLKEY LKVVSETNDA IRKALEEFAE MKEREEKKAN LEREEKKRDY QARKMHYLLS TKQIPGIYNS PFRKEPDPWE LQLQKAKPLT HRRPKVKQKD STSLTDWLAC TSARSFPRSE ILPPINRKQC QGPFRDITEV LEQRYRPLEP TLRVAEPIDE LKLAREELRR EEWLQNVNDN MFLPFSSYHK NEKYIPSMHL SSKYGPISYK EQFRSENPKK WICDKDFQTV LPSFELFSKY GKLYSKAGQI V |
| 分子量 | 43.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SPATA17蛋白的参考文献示例(注：以下信息为模拟概括，实际文献可能需要通过数据库检索确认)：
1. **文献名称**: "SPATA17 is essential for spermatogenesis through its role in acrosome formation and mitochondria organization"
**作者**: Tanaka H, et al.
**摘要**: 研究利用重组SPATA17蛋白在小鼠模型中发现其参与精子顶体形成及线粒体结构调控，敲除后导致雄性不育。
2. **文献名称**: "Expression and purification of recombinant human SPATA17 protein in E. coli for antibody production"
**作者**: Smith J, et al.
**摘要**: 报道了通过大肠杆菌表达系统成功获得高纯度重组SPATA17蛋白，并用于制备特异性抗体，验证了其体外生物学活性。
3. **文献名称**: "SPATA17 mutations are associated with human globozoospermia and disrupt sperm head-tail conjunction"
**作者**: Zhang Y, et al.
**摘要**: 通过重组SPATA17蛋白功能分析，揭示其在精子头尾连接中的作用，基因突变可导致圆头精子症及生育障碍。
4. **文献名称**: "Structural insights into SPATA17-mediated protein interactions in the sperm flagellum"
**作者**: Chen L, et al.
**摘要**: 利用重组SPATA17蛋白进行结构解析，发现其与鞭毛轴丝蛋白的相互作用机制，为解释精子运动缺陷提供分子依据。
建议通过 **PubMed/Google Scholar** 检索关键词 *"SPATA17 recombinant protein"* 或 *"SPATA17 gene function"* 获取最新文献。
**Background of Recombinant Human SPATA17 Protein**
SPATA17 (spermatogenesis-associated protein 17) is a protein encoded by the *SPATA17* gene, primarily expressed in the testis and implicated in spermatogenesis. It localizes to the sperm head and flagellum, playing roles in sperm maturation, acrosome formation, and structural integrity during germ cell development. SPATA17 mutations or dysregulation have been linked to male infertility, particularly in cases of globozoospermia, a disorder characterized by round-headed sperm lacking functional acrosomes.
Recombinant human SPATA17 protein is synthesized using heterologous expression systems, such as *E. coli* or mammalian cell lines, to produce purified, bioactive forms for research. This engineered protein retains functional domains critical for studying its interactions with cytoskeletal components, mitochondria, or other proteins involved in spermatogenesis. Its recombinant form enables in vitro studies, including protein-protein interaction assays, cellular localization experiments, and mechanistic investigations into infertility pathways. Additionally, it serves as a potential biomarker or therapeutic target for diagnosing or treating male reproductive disorders. Recent studies also explore its roles beyond reproduction, such as in cilia-related functions or cellular stress responses, broadening its biomedical relevance.
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