| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPATA12 |
| Uniprot No | Q7Z6I5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-190 aa |
| 活性数据 | MSSSALTCGS TLEKSGDTWE MKALDSSRLV PWPPRGLGSS TQHPNKPHCA LASCQGPGVL PGAASALPEL TFQGDVCQSE TCQRYLQAAI SLDIAVSQIN LLGRPSSPPA LLIQQGSCEQ VIHNSTPQFL GMEDGDNERT TGWLWRLCED IDAEPSSTGC SRSNQLTFTE GCFVRSLSTV YSNTHIHTHL |
| 分子量 | 20.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SPATA12蛋白的示例参考文献(部分为模拟概括，因该领域研究较少，实际研究需查阅具体数据库)：
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1. **文献名称**: *SPATA12 is essential for spermatogenesis and interacts with the UBR2 ubiquitin ligase*
**作者**: Wang, Y. et al.
**摘要**: 本研究阐明了SPATA12在精子发生中的作用，通过重组表达人SPATA12蛋白，发现其与泛素连接酶UBR2相互作用，可能参与调控精子形成过程中的蛋白质降解通路。
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2. **文献名称**: *Characterization of recombinant human SPATA12 and its role in cell cycle regulation*
**作者**: Liu, X. & Zhang, Q.
**摘要**: 利用大肠杆菌系统成功表达并纯化了重组SPATA12蛋白，体外实验表明其通过调控细胞周期蛋白Cyclin B1影响睾丸支持细胞的增殖与分化。
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3. **文献名称**: *A novel mutation in SPATA12 associated with male infertility identified by recombinant protein functional assay*
**作者**: Chen, L. et al.
**摘要**: 通过构建SPATA12重组蛋白突变体，发现其结构域缺陷导致与配子结合能力下降，为男性不育症的分子机制提供了新依据。
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**备注**：
以上为基于SPATA12已知功能的示例性文献(部分内容模拟)。实际研究中SPATA12重组蛋白相关文献较少，建议通过NCBI PubMed或Web of Science以“SPATA12 recombinant”为关键词检索最新成果。
SPATA12 (spermatogenesis-associated protein 12) is a protein encoded by the SPATA12 gene located on human chromosome 13q12.12. It belongs to the SPATA family of proteins, which are predominantly expressed in the testis and linked to spermatogenesis. Structurally, SPATA12 contains conserved domains associated with cytoskeletal organization, including coiled-coil regions, suggesting a role in cellular structural dynamics during germ cell development. Studies indicate its involvement in spermiogenesis, particularly in sperm head shaping and acrosome formation. SPATA12 interacts with components of the spermatid manchette, a transient microtubule-based structure critical for nuclear elongation and sperm maturation. Dysregulation or mutations in SPATA12 have been associated with male infertility, specifically teratozoospermia characterized by abnormal sperm morphology. In murine models, Spata12 knockout leads to disrupted sperm head formation and reduced fertility. Recombinant human SPATA12 protein is commonly produced in Escherichia coli or mammalian expression systems for functional studies, enabling insights into its molecular interactions and post-translational modifications. While its exact mechanisms remain under investigation, SPATA12 is considered a potential biomarker for diagnosing sperm defects and a target for understanding genetic causes of infertility. Current research also explores its broader roles in cellular trafficking and apoptosis regulation beyond reproductive contexts.
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