| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPATA1 |
| Uniprot No | Q5VX52 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-437 aa |
| 活性数据 | MSLNPSRPSS SELVELHVFY VPEGSWNYKL NTISTEVVNK FISAGFLRVS PQLTLRALRE RLGEFLGEDA IAEKFLFLKC IGNNLAVVKE KQESELKLKS FAPPYALQPE LYLLPVMDHL GNVYSPSTVI LDERQTNNGV NEADGTIHRP ISVTLFKEEL GRDPSLLENT LKELPNKNQE EAGGKATAEK SQIAKNQIGN SELPGSLEDS NNDCFGTKKS QCLWENEDDT AISRRQDNQT AEKEYITLPD HPSLPCQPVL SSGITDISLL QTEREKIIKQ MKQVKEERRY LERNREELVK TVEKLFEQSK LKRYHAYNGW KKKYLETKKV TASMEEVLTK LREDLELYYK KLLMQLEARE IKMRPKNLAN ITDSKNYLII QITEVQHAID QLKRKLDTDK MKLIVEVKMR KQAVSDLRTL KTELAQKKKI IHLYNLN |
| 分子量 | 52.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SPATA1蛋白的3篇代表性文献摘要示例(注：部分内容基于现有领域知识推测，实际文献需通过数据库验证)：
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1. **文献名称**: *SPATA1 regulates mitochondrial function in spermatogenesis*
**作者**: Zhang Y, et al.
**摘要**: 本研究首次在大肠杆菌中表达并纯化了重组人SPATA1蛋白，发现其通过调控线粒体膜电位影响精子形成过程中的能量代谢，缺失SPATA1会导致小鼠精子活力下降及男性不育。
2. **文献名称**: *Structural insights into SPATA1’s role in acrosome formation*
**作者**: Li H, Wang X.
**摘要**: 通过X射线晶体学解析了重组人SPATA1蛋白的晶体结构，揭示了其N端结构域与顶体形成相关蛋白的相互作用机制，为解释SPATA1缺陷导致畸形精子的分子机制提供了依据。
3. **文献名称**: *SPATA1 as a diagnostic marker for non-obstructive azoospermia*
**作者**: Chen R, et al.
**摘要**: 利用重组SPATA1蛋白制备多克隆抗体，发现其在非梗阻性无精子症患者睾丸组织中的表达显著下调，提示SPATA1可能作为男性不育的潜在生物标志物。
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**注意**：以上为模拟摘要，实际文献需通过PubMed/Google Scholar检索确认。建议使用关键词 **"SPATA1 recombinant protein"** 或 **"SPATA1 spermatogenesis"** 在学术数据库中查找最新研究。
**Background of Recombinant Human SPATA1 Protein**
SPATA1 (Spermatogenesis-Associated Protein 1) is a poorly characterized protein predominantly expressed in the testis, with putative roles in male reproductive biology. It is encoded by the *SPATA1* gene, located on human chromosome 1p36.13. Although its exact molecular functions remain unclear, SPATA1 is hypothesized to participate in spermatogenesis, particularly in germ cell development, sperm maturation, or structural organization of spermatozoa. Studies suggest its association with the formation of the sperm tail or acrosome, critical for sperm motility and fertilization.
Recombinant human SPATA1 protein is artificially expressed in vitro using biotechnological systems (e.g., bacterial, insect, or mammalian cell lines) to enable functional and structural studies. Its recombinant form allows researchers to investigate its interactions with other proteins, enzymatic activities (if any), and potential involvement in signaling pathways. Emerging evidence links *SPATA1* mutations or dysregulation to male infertility, underscoring its clinical relevance. However, mechanistic insights into its role in cellular processes, such as cytoskeletal dynamics or intracellular transport, are still limited.
Current research focuses on elucidating SPATA1’s 3D structure, binding partners, and regulatory mechanisms. Its recombinant production also supports antibody development for diagnostic or therapeutic applications in reproductive disorders. Despite progress, SPATA1 remains an understudied protein, warranting further exploration to unravel its contributions to spermatogenesis and broader physiological contexts.
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