| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPATC1 |
| Uniprot No | Q76KD6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-591aa |
| 活性数据 | MSLLTNYEGL RHQIERLVRE NEELKKLVRL IRENHELKSA IKTQAGGLGI SGFTSGLGEA TAGLSSRQNN GVFLPPSPAV ANERVLEEVG IMALAPLAEM LTSLQPSATP GSLMSPLTGT LSTLLSGPAP TSQSSPLTSF LTSPIAGPLT GTLASSLGLP STGTLTPSSL VAGPVAMSQS SPLIAPVMGT VAVSLSSPLL SSTATPPGVS QNLLANPMSN LVLPEAPRLR LAEPLRGGPT GPQSPACVVP TATTKVPLST EPPQSTQDPE PLSMAFAGAP LQTSTPIGAM GTPAPKTAFS FNTSDTQAQP SAAQEQVVPA SVPTSPTTSP TVTVLASAPA LAPQVATSYT PSSTTHIAQG APHPPSRMHN SPTQNLPVPH CPPHNAHSPP RTSSSPASVN DSRGPRTTEP STKSMMEVER KLAHRKTSKF PENPRESKQL AWERLVGEIA FQLDRRILSS IFPERVRLYG FTVSNIPEKI IQASLNPSDH KLDEKLCQRL TQRYVSVMNR LQSLGYNGRV HPALTEQLVN AYGILRERPE LAASEGGPYT VDFLQRVVVE TVHPGMLADA LLLLSCLSQL AHDDGKPMFI W |
| 分子量 | 62.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与SPATC1蛋白相关的代表性文献摘要(均为虚构示例，实际文献需检索数据库)：
1. **《SPATC1 regulates spermatid head shaping in mice》**
- 作者：Chen Y, et al.
- 摘要：研究揭示了SPATC1在小鼠精子形成中的关键作用，发现其通过与细胞骨架蛋白相互作用调控精子头部的形态发育，敲除SPATC1导致雄性不育。
2. **《Crystal structure of human SPATC1 reveals a centrosome-targeting domain》**
- 作者：Tanaka K, et al.
- 摘要：首次解析了人源SPATC1蛋白的晶体结构，发现其C端具有中心体定位结构域，为研究其在细胞分裂中的功能提供结构基础。
3. **《SPATC1 overexpression correlates with poor prognosis in hepatocellular carcinoma》**
- 作者：Wang L, et al.
- 摘要：通过免疫组化分析发现，SPATC1在肝细胞癌中异常高表达，并通过调控Wnt/β-catenin通路促进肿瘤转移，提示其作为癌症生物标志物的潜力。
**注**：以上内容为模拟生成，实际研究中建议通过PubMed/Google Scholar检索“SPATC1”或“sperm-associated antigen 6”获取真实文献(SPATC1可能与生殖及癌症研究相关)。如需具体文献，请提供数据库访问权限。
SPATC1 (Spermatogenesis and Centriole-Associated Protein 1) is a human protein encoded by the *SPATC1* gene, primarily studied for its role in germ cell development and centriole-related functions. It is predominantly expressed in the testis, particularly during spermatogenesis, where it localizes to the centrosome and manchette structure in developing sperm. SPATC1 is implicated in regulating centriole duplication, sperm head shaping, and chromosomal segregation, with defects linked to male infertility and teratozoospermia. Structurally, it contains conserved coiled-coil domains that mediate protein-protein interactions, critical for its role in cytoskeletal organization and cell division.
Recombinant human SPATC1 protein is typically produced using expression systems like *E. coli* or mammalian cells, often fused with tags (e.g., His, GST) for purification and detection. Its recombinant form enables in vitro studies to dissect molecular mechanisms, such as binding partners (e.g., tubulin, centrosomal proteins) and post-translational modifications. Researchers utilize it to explore pathologies like azoospermia, ciliopathies, or cancers with centrosomal abnormalities. Additionally, it aids in developing diagnostic tools or therapies targeting spermatogenic defects. Current research focuses on elucidating SPATC1's interplay with meiosis regulators and its potential as a biomarker for fertility assessment.
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