| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPATA5 |
| Uniprot No | Q8NB90 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-695 aa |
| 活性数据 | MSSKKNRKRLNQSAENGSSLPSAASSCAEARAPSAGSDFAATSGTLTVTNLLEKDDKIPKTFQNSLIHLGLNTMKSANICIGRPVLLTSLNGKQEVYTAWPMAGFPGGKVGLSEMAQKNVGVRPGDAIQVQPLVGAVLQAEEMDVALSDKDMEINEEELTGCILRKLDGKIVLPGNFLYCTFYGRPYKLQVLRVKGADGMILGGPQSDSDTDAQRMAFEQSSMETSSLELSLQLSQLDLEDTQIPTSRSTPYKPIDDRITNKASDVLLDVTQSPGDGSGLMLEEVTGLKCNFESAREGNEQLTEEERLLKFSIGAKCNTDTFYFISSTTRVNFTEIDKNSKEQDNQFKVTYDMIGGLSSQLKAIREIIELPLKQPELFKSYGIPAPRGVLLYGPPGTGKTMIARAVANEVGAYVSVINGPEIISKFYGETEAKLRQIFAEATLRHPSIIFIDELDALCPKREGAQNEVEKRVVASLLTLMDGIGSEVSEGQVLVLGATNRPHALDAALRRPGRFDKEIEIGVPNAQDRLDILQKLLRRVPHLLTEAELLQLANSAHGYVGADLKVLCNEAGLCALRRILKKQPNLPDVKVAGLVKITLKDFLQAMNDIRPSAMREIAIDVPNVSWSDIGGLESIKLKLEQAVEWPLKHPESFIRMGIQPPKGVLLYGPPGCSKTMIAKALANESGLNFLAIKVGC |
| 分子量 | 102 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SPATA5蛋白的3篇文献示例(基于已知研究方向和假设性摘要概括)：
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1. **文献名称**: *SPATA5 regulates neuronal mitochondrial function and is linked to neurodevelopmental disorders*
**作者**: Johnson, T. et al. (2020)
**摘要**: 研究通过重组表达人SPATA5蛋白，发现其具有ATP依赖的酶活性，且在神经元线粒体功能中起关键作用。SPATA5突变体的重组蛋白活性显著降低，与患者中的癫痫和智力障碍表型相关，提示其在神经发育中的作用。
2. **文献名称**: *Crystal structure of the human SPATA5 protein reveals a conserved ATPase domain*
**作者**: Thompson, R. et al. (2019)
**摘要**: 报道了重组SPATA5蛋白的晶体结构解析，揭示了其核心ATP酶结构域与其他AAA+蛋白家族的相似性，为突变导致细胞分裂异常(如染色体分离缺陷)的机制提供了结构基础。
3. **文献名称**: *SPATA5 deficiency disrupts mitotic progression via impaired centrosomal dynamics*
**作者**: Suzuki, K. et al. (2017)
**摘要**: 利用重组SPATA5蛋白进行体外实验，证明其通过调控中心体微管组织功能参与有丝分裂进程。SPATA5敲除细胞中补充重组蛋白可恢复分裂异常，表明其直接参与细胞周期调控。
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**注意事项**：
- 上述文献为示例，实际研究可能存在差异，建议通过PubMed等数据库以“SPATA5 recombinant”或“SPATA5 ATPase”为关键词检索最新原文。
- SPATA5在疾病中的研究较多聚焦于基因突变(如神经发育障碍)，重组蛋白研究可能较少，建议扩展至其相互作用蛋白或通路分析。
SPATA5 (Spermatogenesis-Associated Protein 5) is a conserved eukaryotic protein encoded by the SPATA5 gene, initially linked to spermatogenesis but later found to play broader roles in cellular functions. It is implicated in mitochondrial dynamics, energy metabolism, and cell cycle regulation, particularly during mitosis. Structurally, SPATA5 contains a conserved ATPase domain and putative substrate-binding motifs, suggesting roles in ATP-dependent processes like protein trafficking or complex assembly. Its dysfunction is associated with neurodevelopmental disorders, including epilepsy, intellectual disability, and hearing loss, as seen in SPATA5-linked syndrome caused by recessive mutations. Studies also highlight its involvement in mitochondrial quality control and neuronal survival, possibly through interactions with proteins involved in oxidative phosphorylation or apoptosis. Recombinant human SPATA5 protein, produced via bacterial or mammalian expression systems, enables functional studies to dissect mechanistic pathways and explore therapeutic strategies. Research focuses on its interplay with mitochondrial proteins, impact on synaptic function, and potential as a biomarker for neurodevelopmental conditions. Despite progress, its precise molecular mechanisms remain under investigation, underscoring the need for further structural and functional characterization.
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