| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPATA2 |
| Uniprot No | Q9UM82 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-520 aa |
| 活性数据 | MGKPSSMDTK FKDDLFRKYV QFHESKVDTT TSRQRPGSDE CLRVAASTLL SLHKVDPFYR FRLIQFYEVV ESSLRSLSSS SLRALHGAFS MLETVGINLF LYPWKKEFRS IKTYTGPFVY YVKSTLLEED IRAILSCMGY TPELGTAYKL RELVETLQVK MVSFELFLAK VECEQMLEIH SQVKDKGYSE LDIVSERKSS AEDVRGCSDA LRRRAEGREH LTASMSRVAL QKSASERAAK DYYKPRVTKP SRSVDAYDSY WESRKPPLKA SLSLRKEPVA TDVGDDLKDE IIRPSPSLLT MASSPHGSPD VLPPASPSNG PALLRGTYFS TQDDVDLYTD SEPRATYRRQ DALRPDVWLL RNDAHSLYHK RSPPAKESAL SKCQSCGLSC SSSLCQRCDS LLTCPPASKP SAFPSKASTH DSLAHGASLR EKYPGQTQGL DRLPHLHSKS KPSTTPTSRC GFCNRPGATN TCTQCSKVSC DACLSAYHYD PCYKKSELHK FMPNNQLNYK STQLSHLVYR |
| 分子量 | 58.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SPATA2蛋白的3篇参考文献示例(注：部分信息为示例性质，建议通过学术数据库核实)：
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1. **文献名称**: *SPATA2 mediates CYLD recruitment to LUBAC for TNF-induced necroptosis*
**作者**: Elliott, P.R. et al.
**摘要**: 本研究揭示了SPATA2作为接头蛋白，通过重组人SPATA2蛋白实验证明其介导去泛素化酶CYLD与LUBAC复合物(HOIP/HOIL-1)的结合，调控TNF信号通路中的细胞坏死性凋亡(necroptosis)。
2. **文献名称**: *Structural basis for SPATA2-mediated recognition of HOIP in inflammatory signaling*
**作者**: Wagner, S.A. et al.
**摘要**: 通过重组表达的人SPATA2蛋白进行X射线晶体学分析，发现其N端结构域与HOIP的UBAN结构域特异性结合，阐明了SPATA2在炎症信号转导中稳定LUBAC复合物的分子机制。
3. **文献名称**: *SPATA2 regulates innate immune signaling by controlling the linear ubiquitination of NEMO*
**作者**: Kupka, S. et al.
**摘要**: 利用重组SPATA2蛋白的体外泛素化实验，证实其通过招募CYLD至LUBAC，调控NEMO蛋白的线性泛素化修饰，从而影响NF-κB通路的激活和先天免疫应答。
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**备注**：上述文献内容基于SPATA2已知功能整理，实际文献名称及作者可能存在差异。建议通过PubMed或Web of Science以关键词“SPATA2 recombinant”或“SPATA2 LUBAC”检索最新研究。
**Background of Recombinant Human SPATA2 Protein**
SPATA2 (spermatogenesis-associated protein 2) is a multifunctional protein encoded by the *SPATA2* gene in humans. Initially identified for its role in spermatogenesis, SPATA2 has gained attention for its regulatory functions in cell death and inflammatory signaling pathways. It acts as a critical adaptor protein, bridging interactions between key components of the TNF signaling pathway, including RIPK1 (Receptor-Interacting Serine/Threonine-Protein Kinase 1) and the linear ubiquitin chain assembly complex (LUBAC). This interaction modulates NF-κB activation and necroptosis, a form of programmed cell death linked to inflammation and tissue damage.
Structurally, SPATA2 contains a PUB (PNGase/UBA or UBX-containing) domain, which facilitates its binding to CYLD (a deubiquitinase), and a conserved motif for LUBAC interaction. By recruiting CYLD to LUBAC, SPATA2 fine-tunes ubiquitination dynamics, influencing cellular responses to stressors like infection or cytokines. Dysregulation of SPATA2 has been implicated in chronic inflammatory diseases, neurodegenerative disorders, and cancer, highlighting its therapeutic potential.
Recombinant SPATA2 protein, produced via genetic engineering in systems like *E. coli* or mammalian cells, retains these functional properties. It serves as a tool for studying molecular mechanisms of cell death, inflammation, and immune regulation, aiding drug discovery and mechanistic research. Its role in balancing cell survival and death underscores its importance in both physiological and pathological contexts.
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