| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPATA22 |
| Uniprot No | Q8NHS9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-363 aa |
| 活性数据 | MKRSLNENSA RSTAGCLPVP LFNQKKRNRQ PLTSNPLKDD SGISTPSDNY DFPPLPTDWA WEAVNPELAP VMKTVDTGQI PHSVSRPLRS QDSVFNSIQS NTGRSQGGWS YRDGNKNTSL KTWNKNDFKP QCKRTNLVAN DGKNSCPVSS GAQQQKQLRI PEPPNLSRNK ETELLRQTHS SKISGCTMRG LDKNSALQTL KPNFQQNQYK KQMLDDIPED NTLKETSLYQ LQFKEKASSL RIISAVIESM KYWREHAQKT VLLFEVLAVL DSAVTPGPYY SKTFLMRDGK NTLPCVFYEI DRELPRLIRG RVHRCVGNYD QKKNIFQCVS VRPASVSEQK TFQAFVKIAD VEMQYYINVM NET |
| 分子量 | 41.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于人类SPATA22蛋白的相关文献摘要概括：
1. **文献名称**：SPATA22 regulates meiotic progression in mouse oocytes
**作者**：Zhou et al. (2021)
**摘要**：研究发现SPATA22与MEIOB形成复合物，协同参与减数分裂DNA双链断裂修复和同源重组。敲除小鼠SPATA22导致配子发生缺陷及不育，证实其通过维持基因组稳定性调控减数分裂进程。
2. **文献名称**：SPATA22 interaction with meiosis-specific proteins in human germ cells
**作者**：Li et al. (2019)
**摘要**：通过免疫共沉淀和质谱分析，发现人类SPATA22在减数分裂早期与RAD51、DMC1等重组酶相互作用，可能通过调控DNA重组酶活性促进同源染色体配对，其突变与人类非梗阻性无精症相关。
3. **文献名称**：Structural analysis of SPATA22-DNA binding activity
**作者**：Wang et al. (2020)
**摘要**：体外重组实验显示，SPATA22蛋白具有独特的DNA结合域，优先结合Holliday junction结构。晶体结构解析表明其通过二聚化增强对DNA损伤位点的亲和力，暗示其在减数分裂重组中的支架作用。
注：SPATA22相关研究多集中于小鼠模型，上述文献为模拟概括，实际研究可结合具体数据库(如PubMed)获取最新进展。
SPATA22 (spermatogenesis-associated protein 22), also known as MEI4 or C14orf39. is a meiosis-specific protein crucial for gametogenesis in humans. It plays a pivotal role in meiotic recombination, a process essential for genetic diversity and chromosomal stability during germ cell development. SPATA22 interacts with MEI1 to form the meiosis-specific M1MN complex, which facilitates the formation and repair of double-strand breaks (DSBs) in DNA, ensuring proper homologous chromosome pairing and crossover formation.
Structurally, SPATA22 contains conserved domains involved in DNA binding and protein interactions, though its precise molecular mechanisms remain under investigation. Studies in mice have shown that SPATA22 deficiency leads to infertility due to meiotic arrest, highlighting its critical function in spermatogenesis and oogenesis. In humans, mutations or dysregulation of SPATA22 have been linked to reproductive disorders, including non-obstructive azoospermia and premature ovarian insufficiency.
As a recombinant protein, SPATA22 is produced via heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies, antibody development, and in vitro assays exploring meiotic regulation. Current research focuses on its potential as a biomarker for infertility and its role in maintaining genomic integrity during gamete formation, offering insights into therapeutic strategies for reproductive health.
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