| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPATA3 |
| Uniprot No | Q8NHX4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-183 aa |
| 活性数据 | MKKVKKKRSE ARRHRDSTSQ HASSNSTSQQ PSPESTPQQP SPESTPQHSS LETTSRQPAF QALPAPEIRR SSCCLLSPDA NVKAAPQSRK AGPLIRAGPH SCSCATCPCS SACWRRLGLC HSRIFDVLLP RDWQMAPGRG LPNLLTFYRK SSRKPSSHRN ACPPSPRNCG CGSGGSRSCL LHH |
| 分子量 | 20.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SPATA3蛋白的3篇示例文献，基于研究方向的合理假设(需通过学术数据库验证具体内容)：
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1. **文献名称**：*SPATA3 regulates spermatogenesis through interaction with HDAC4 in mouse testis*
**作者**：Li Y, Zhang R, et al.
**摘要**：本研究阐明了SPATA3在小鼠精子发生中的功能，发现其通过结合组蛋白去乙酰化酶HDAC4调控靶基因表达，并通过重组表达SPATA3蛋白验证了其在生殖细胞凋亡中的保护作用。
2. **文献名称**：*Structural characterization and functional analysis of recombinant human SPATA3 protein*
**作者**：Wang X, Liu T, et al.
**摘要**：首次报道了重组人SPATA3蛋白在大肠杆菌中的可溶性表达及纯化方法，并解析了其晶体结构，揭示了SPATA3中特有的功能结构域与潜在的蛋白质结合位点。
3. **文献名称**：*SPATA3 promotes apoptosis in ovarian cancer cells via modulating mitochondrial pathways*
**作者**：Chen L, Sun H, et al.
**摘要**：发现SPATA3在卵巢癌细胞中过表达可通过激活线粒体途径诱导凋亡，体外实验中利用重组SPATA3蛋白证实其对癌细胞增殖的抑制作用，提示其作为癌症治疗的潜在靶点。
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**注意**：以上文献为示例，实际研究需通过PubMed、Web of Science等平台检索关键词(如“SPATA3 recombinant protein”或“SPATA3 function”)，并建议优先选择近5年发表的论文以获取最新进展。
SPATA3 (Spermatogenesis Associated Protein 3) is a protein encoded by the SPATA3 gene, primarily expressed in the testis and linked to reproductive biology. It belongs to the SPATA family of proteins, which are implicated in spermatogenesis, though its exact molecular mechanisms remain partially understood. Studies suggest SPATA3 localizes to the cytoplasm and nucleus, potentially playing roles in cell cycle regulation, apoptosis, or oxidative stress response. Notably, it interacts with apoptosis-related proteins like APAF1. hinting at involvement in caspase-dependent apoptotic pathways. Dysregulation of SPATA3 has been associated with male infertility and certain cancers, including testicular and ovarian malignancies.
Recombinant human SPATA3 protein is produced using expression systems like E. coli or mammalian cells, often fused with tags (e.g., His-tag) for purification and detection. This engineered protein enables functional studies, antibody development, and screening for molecular interactions. Researchers utilize it to explore SPATA3’s role in germ cell development, DNA damage repair, and its potential as a biomarker for reproductive disorders. Current challenges include elucidating its full range of binding partners and resolving structural complexities. Despite gaps in mechanistic understanding, SPATA3 remains a focus for reproductive health research and therapeutic target exploration.
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