| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPATA5L1 |
| Uniprot No | Q9BVQ7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-753aa |
| 活性数据 | MAPDSDPFPE GPLLKLLPLD ARDRGTQRCR LGPAALHALG ARLGSAVKIS LPDGGSCLCT AWPRRDGADG FVQLDPLCAS PGAAVGASRS RRSLSLNRLL LVPCPPLRRV AVWPVLRERA GAPGARNTAA VLEAAQELLR NRPISLGHVV VAPPGAPGLV AALHIVGGTP SPDPAGLVTP RTRVSLGGEP PSEAQPQPEV PLGGLSEAAD SLRELLRLPL RYPRALTALG LAVPRGVLLA GPPGVGKTQL VRAVAREAGA ELLAVSAPAL QGSRPGETEE NVRRVFQRAR ELASRGPSLL FLDEMDALCP QRGSRAPESR VVAQVLTLLD GASGDREVVV VGATNRPDAL DPALRRPGRF DREVVIGTPT LKQRKEILQV ITSKMPISSH VDLGLLAEMT VGYVGADLTA LCREAAMHAL LHSEKNQDNP VIDEIDFLEA FKNIQPSSFR SVIGLMDIKP VDWEEIGGLE DVKLKLKQSI EWPLKFPWEF VRMGLTQPKG VLLYGPPGCA KTTLVRALAT SCHCSFVSVS GADLFSPFVG DSEKVLSQIF RQARASTPAI LFLDEIDSIL GARSASKTGC DVQERVLSVL LNELDGVGLK TIERRGSKSS QQEFQEVFNR SVMIIAATNR PDVLDTALLR PGRLDKIIYI PPPDHKGRLS ILKVCTKTMP IGPDVSLENL AAETCFFSGA DLRNLCTEAA LLALQENGLD ATTVKQEHFL KSLKTVKPSL SCKDLALYEN LFKKEGFSNV EGI |
| 分子量 | 80.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人SPATA5L1蛋白的模拟参考文献(注：以下内容为示例，实际文献请通过学术数据库查询)：
1. **文献名称**: *Structural characterization of human SPATA5L1 and its interaction with mitochondrial proteins*
**作者**: Chen L, et al.
**摘要**: 通过X射线晶体学解析了重组人SPATA5L1蛋白的ATP酶结构域三维结构，并发现其通过与线粒体膜蛋白TOMM20的相互作用参与线粒体功能调控。
2. **文献名称**: *SPATA5L1 deficiency disrupts spermatogenesis through aberrant chromatin remodeling*
**作者**: Tanaka K, et al.
**摘要**: 研究利用CRISPR/Cas9构建的SPATA5L1敲除小鼠模型，发现该蛋白缺失导致精子发生异常，与组蛋白修饰酶复合体结合并参与染色质重构。
3. **文献名称**: *Proteomic profiling identifies SPATA5L1 as a novel biomarker in hepatocellular carcinoma*
**作者**: Wang Y, et al.
**摘要**: 通过肝癌组织蛋白质组学分析发现SPATA5L1显著高表达，体外实验证实其过表达促进肿瘤细胞增殖，可能与Hippo信号通路抑制相关。
建议通过PubMed或Google Scholar检索关键词“SPATA5L1”或“SPATA5-like protein 1”获取真实文献。
Recombinant human SPATA5L1 (spermatogenesis-associated protein 5-like 1) is a less-characterized protein encoded by the SPATA5L1 gene, belonging to the SPATA5 protein family. Though structurally related to SPATA5—a protein implicated in mitochondrial function, spermatogenesis, and neurodevelopment—the precise biological role of SPATA5L1 remains unclear. It shares conserved ATPase domains, suggesting potential involvement in energy-dependent processes like protein folding, vesicle trafficking, or chromatin remodeling.
SPATA5L1 is ubiquitously expressed across tissues, with elevated levels detected in the brain, testes, and kidneys. Its recombinant form is typically produced in Escherichia coli or mammalian expression systems for functional studies. Emerging evidence links SPATA5L1 to neurodevelopmental disorders, as chromosomal deletions encompassing this gene are associated with microcephaly, hearing loss, and intellectual disability. In vitro studies suggest its interaction with mitochondrial proteins, hinting at roles in mitochondrial quality control or apoptosis regulation.
Research challenges include its low basal expression and functional redundancy with SPATA5. Current investigations focus on elucidating its interactome, post-translational modifications, and disease-associated mutations. As a recombinant tool, SPATA5L1 enables exploration of its enzymatic properties and pathologic mechanisms, potentially paving the way for therapeutic strategies in related genetic disorders. Further studies are needed to define its molecular pathways and tissue-specific functions.
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