| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPC24 |
| Uniprot No | Q8NBT2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-197aa |
| 活性数据 | MAAFRDIEEV SQGLLSLLGA NRAEAQQRRL LGRHEQVVER LLETQDGAEK QLREILTMEK EVAQSLLNAK EQVHQGGVEL QQLEAGLQEA GEEDTRLKAS LLYLTRELEE LKEIEADLER QEKEVDEDTT VTIPSAVYVA QLYHQVSKIE WDYECEPGMV KGIHHGPSVA QPIHLDSTQL SRKFISDYLW SLVDTEW |
| 分子量 | 22.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SPC24蛋白的3篇参考文献示例(注：内容为模拟生成，仅供参考)：
1. **文献名称**: *"Functional characterization of recombinant human SPC24 in the Ndc80 complex during mitosis"*
**作者**: Miller KL, et al.
**摘要**: 本研究通过在大肠杆菌中表达并纯化重组人SPC24蛋白，探究其与Ndc80复合体其他亚基的相互作用。实验表明SPC24对复合体的微管结合能力至关重要，敲低SPC24会导致染色体排列异常。
2. **文献名称**: *"Structural insights into the SPC24/SPC25 heterodimer of the human Ndc80 complex"*
**作者**: Chen Z, et al.
**摘要**: 利用X射线晶体学解析了重组SPC24/SPC25异源二聚体的三维结构，揭示了其与着丝粒结合的分子机制，为靶向该复合体的抗癌药物设计提供了结构基础。
3. **文献名称**: *"Expression and purification of recombinant human SPC24 for antibody production"*
**作者**: Gupta R, et al.
**摘要**: 描述了一种在昆虫细胞SF9系统中高效表达重组人SPC24蛋白的策略，通过亲和层析获得高纯度蛋白，并成功用于开发特异性抗体，验证了其在乳腺癌细胞中的定位。
(注：实际文献需通过PubMed/Google Scholar等平台以关键词“SPC24”、“Ndc80 complex”、“recombinant”检索核实。)
SPC24 is a key protein involved in mitotic processes, specifically as a component of the KNL1/MTW1 complex within the outer kinetochore. It interacts with SPC25 to form part of the Ndc80 complex, which is critical for proper chromosome segregation during cell division by linking kinetochores to spindle microtubules. This interaction ensures accurate microtubule attachment, spindle assembly checkpoint signaling, and alignment of chromosomes during metaphase. Dysregulation of SPC24 can lead to mitotic errors, aneuploidy, and genomic instability, which are hallmarks of cancers and developmental disorders. Recombinant human SPC24 protein is typically produced using bacterial (e.g., *E. coli*) or mammalian expression systems, enabling studies on its structural and functional roles in vitro. Its applications include investigating kinetochore-microtubule dynamics, screening for anti-mitotic drugs, and exploring mechanisms underlying chromosome missegregation. Structural studies using recombinant SPC24. often combined with techniques like X-ray crystallography or cryo-EM, have shed light on its conserved helical domains and binding interfaces with other kinetochore components. Research on SPC24 also contributes to understanding cancer pathogenesis, as its overexpression or mutations may serve as potential therapeutic targets to disrupt aberrant cell proliferation.
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