| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPC25 |
| Uniprot No | Q9HBM1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-224 aa |
| 活性数据 | MVEDELALFDKSINEFWNKFKSTDTSCQMAGLRDTYKDSIKAFAEKLSVKLKEEERMVEMFLEYQNQISRQNKLIQEKKDNLLKLIAEVKGKKQELEVLTANIQDLKEEYSRKKETISTANKANAERLKRLQKSADLYKDRLGLEIRKIYGEKLQFIFTNIDPKNPESPFMFSLHLNEARDYEVSDSAPHLEGLAEFQENVRKTNNFSAFLANVRKAFTATVYN |
| 分子量 | 52.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于重组人SPC25蛋白的假设性参考文献(注：部分内容可能根据实际研究背景模拟概括，建议通过PubMed或Web of Science查询最新文献)：
1. **文献名称**：*Structural insights into the human Ndc80 complex and its interaction with SPC25*
**作者**：Smith J, et al.
**摘要**：通过重组表达人SPC25蛋白与Ndc80复合体其他亚基，解析了该复合体在动粒微管结合中的三维结构，揭示了SPC25与HEC1的相互作用对染色体分离的调控机制。
2. **文献名称**：*Recombinant SPC25 regulates kinetochore localization and mitotic progression in vitro*
**作者**：Li X, et al.
**摘要**：利用大肠杆菌系统表达并纯化重组人SPC25蛋白，通过体外实验证明其与微管的结合能力缺失会导致有丝分裂中染色体排列异常，提示其作为癌症治疗靶点的潜力。
3. **文献名称**：*Functional characterization of SPC25 phosphorylation in the DNA damage response*
**作者**：Wang Y, et al.
**摘要**：研究重组SPC25蛋白的磷酸化修饰位点，发现其在DNA损伤应答中通过影响Ndc80复合体稳定性参与细胞周期检查点调控。
如需具体文献，可补充说明研究方向(如结构、疾病关联或表达技术)，进一步辅助筛选。
SPC25 is a critical component of the Ndc80 complex (also known as the KMN network), a conserved protein assembly essential for accurate chromosome segregation during mitosis. It functions at kinetochores, serving as a bridge between microtubules and chromosomal centromeres to ensure proper spindle attachment and faithful chromosome distribution. Structurally, SPC25 interacts with SPC24 to form a stable heterodimer that anchors the Ndc80 complex to kinetochores, facilitating microtubule-binding activity through the N-terminal regions of Ndc80 and Nuf2 subunits. This interaction is vital for activating the spindle assembly checkpoint (SAC), a surveillance mechanism that delays anaphase until all chromosomes achieve bipolar attachment. Dysregulation of SPC25 compromises genomic stability, leading to aneuploidy and chromosomal missegregation, hallmarks of cancer and developmental disorders. Recombinant human SPC25 protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), retains native folding and interaction properties. It is widely used in biochemical studies to dissect kinetochore-microtubule dynamics, reconstitute SAC signaling in vitro, and screen for therapeutic agents targeting mitotic errors. Mutational analyses of recombinant SPC25 have also clarified its role in oncogenesis and chemotherapy resistance. Research on this protein provides insights into cell division mechanics and potential strategies for cancer treatment.
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