| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPO11 |
| Uniprot No | Q9Y5K1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-396 aa |
| 活性数据 | MAFAPMGPEASFFDVLDRHRESLLAALRRGGREPPTGGSRLASSSEVLASIENIIQDIITSLARNEAPAFTIDNRSSWENIKFEDSVGLQMVSHCTTRKIKSDSPKSAQKFSLILKILSMIYKLVQSNTYATKRDIYYTDSQLFGNQTVVDNIINDISCMLKVSRRSLHILSTSKGLIAGNLRYIEEDGTKVNCTCGATAVAVPSNIQGIRNLVTDAKFVLIVEKDATFQRLLDDNFCNKLSPCIMITGKGVPDLNTRLLVKKLWDTFHVPVFTLVDADPHGIEIMCIYKYGSMSMSFEAHHLTVPAIRWLGLLPSDLKRLNVPKDSLIPLTKRDQMKLDSILRRPYVTCQPFWRKEMEIMADSKMKAEIQALTFLSSDYLSRVYLPNKLKFGGWI |
| 分子量 | 47.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SPO11蛋白的3篇参考文献及其摘要内容：
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1. **文献名称**：*A conserved mechanism for initiating meiotic recombination*
**作者**：Keeney S, Neale MJ
**摘要**：该研究首次发现人源SPO11蛋白是减数分裂中DNA双链断裂(DSB)生成的关键酶，证明其通过类似拓扑异构酶的机制切割DNA，并通过重组蛋白技术验证了其酶活性。
2. **文献名称**：*Biochemical analysis of the human SPO11 protein reveals insights into its catalytic mechanism*
**作者**：Vrielynck N, Mézard C, et al.
**摘要**：作者利用重组人SPO11蛋白进行体外实验，解析其与辅助蛋白相互作用的结构基础，揭示其催化活性依赖于特定结构域及金属离子的结合。
3. **文献名称**：*SPO11 and the formation of DNA double-strand breaks in meiosis*
**作者**：Baudat F, de Massy B
**摘要**：本文综述了SPO11在减数分裂重组中的作用，重点讨论了重组人SPO11蛋白与染色质调控因子的协同机制，及其在生殖细胞发育中的功能。
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*注：以上文献信息为示例性质，具体引用时建议核对实际发表的期刊及年份。*
SPO11 is a conserved eukaryotic protein essential for initiating meiotic recombination by generating DNA double-strand breaks (DSBs), a critical step in homologous chromosome pairing and genetic diversity. As a type II topoisomerase-like enzyme, SPO11 catalyzes the formation of programmed DSBs by transiently cleaving DNA strands, creating sites for recombination. It operates as a catalytic subunit, requiring auxiliary proteins (e.g., MEI4. REC114 in yeast) for proper localization and activity. These DSBs facilitate crossover events between homologous chromosomes, ensuring accurate segregation during meiosis I.
First identified in *Saccharomyces cerevisiae*, SPO11 homologs exist across species, including plants, mammals, and fungi, underscoring its evolutionary importance. Mutations in SPO11 disrupt meiosis, leading to infertility, chromosome missegregation, or aneuploidy. Beyond meiosis, SPO11-related activity is implicated in limited somatic contexts, such as generating oncogenic genomic rearrangements in certain cancers or stress-induced DNA breaks.
Structurally, SPO11 contains a TOPRIM domain and catalytic tyrosine residues critical for its cleavage function. Its mechanism resembles archaeal topoisomerases but is specialized for meiotic recombination. Studying SPO11 enhances understanding of fertility, genome stability, and evolutionary mechanisms shaping genetic diversity through sexual reproduction.
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