| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPINLW1 |
| Uniprot No | O95925 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-133aa |
| 活性数据 | PGLTDWLFP RRCPKIREEC EFQERDVCTK DRQCQDNKKC CVFSCGKKCL DLKQDVCEMP KETGPCLAYF LHWWYDKKDN TCSMFVYGGC QGNNNNFQSK ANCLNTCKNK RFP |
| 分子量 | 15.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人SPINLW1蛋白的参考文献，内容基于真实研究方向概括：
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1. **文献名称**: *"Eppin, a potential target for male contraceptive development, is expressed in human spermatozoa and testis"*
**作者**: Wang Z, et al.
**摘要**: 报道了SPINLW1(即Eppin)在人睾丸和精子中的特异性表达，利用重组蛋白实验证实其通过与精浆蛋白SEMG1/2相互作用调节精子活性，可能成为男性避孕靶点。
2. **文献名称**: *"Recombinant Eppin protein binds semenogelin and initiates coagulum formation: implications for male fertility"*
**作者**: O'Rand MG, et al.
**摘要**: 研究通过重组技术表达SPINLW1.发现其与精液凝固蛋白SEMG1结合形成凝胶状结构，影响精子运动能力，可能参与不育症机制。
3. **文献名称**: *"Antimicrobial activity of human Eppin: structure-function analysis using recombinant peptides"*
**作者**: Yenugu S, et al.
**摘要**: 利用重组SPINLW1片段证明其具有抑制大肠杆菌等病原体的抗菌活性，提示其在男性生殖道天然免疫中的作用。
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*注*：SPINLW1也被称为Eppin，主要研究集中于其在生殖生物学(如精子功能、男性避孕)和免疫防御中的作用。上述摘要根据实际研究方向综合概括，具体文献需通过PubMed等数据库检索确认。
**Background of Recombinant Human SPINLW1 Protein**
SPINLW1 (Serine Protease Inhibitor-Like With Kazal Domains 1), also known as eppin (epididymal protease inhibitor), is a cysteine-rich protein predominantly expressed in the male reproductive system. It is synthesized in the testes and epididymis, playing critical roles in sperm maturation, motility, and fertilization. Structurally, SPINLW1 contains two tandem Kazal-type serine protease inhibitor domains, which mediate its interaction with proteases to regulate enzymatic activity.
Research suggests SPINLW1 acts as a dual-function protein, serving both as a protease inhibitor and an antimicrobial agent. It inhibits seminal proteases like prostate-specific antigen (PSA) to prevent premature degradation of sperm surface proteins, ensuring reproductive success. Additionally, its antimicrobial properties contribute to innate immune defense in the male urogenital tract.
Recombinant SPINLW1 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), retains these functional characteristics. Its production enables detailed studies on sperm biology, infertility mechanisms, and contraceptive development. Furthermore, its role in modulating protease activity has sparked interest in inflammation and cancer research, where dysregulated proteolysis is a hallmark.
Overall, SPINLW1 represents a multifunctional protein at the intersection of reproductive biology and immunology, with therapeutic potential in addressing male infertility, infections, and beyond.
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