| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SURF5 |
| Uniprot No | Q15528 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-140 aa |
| 活性数据 | MAQQRALPQSKETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIEDETQVSRATQGEQDNYEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRNQQLRTLQEECDRKLITLRDEISIDLYELEEEYYSSRYK |
| 分子量 | 42.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SURF5蛋白的参考文献示例(部分为模拟文献，供参考)：
1. **"Structural and functional analysis of the SURF5 protein in the human SURF gene cluster"**
**作者**: Smith, J. et al. (2005)
**摘要**: 本研究解析了SURF5在SURF基因簇中的定位，并通过重组表达技术在大肠杆菌中制备了SURF5蛋白，验证其RNA结合活性，为研究其在RNA代谢中的作用提供依据。
2. **"Recombinant expression of SURF5 in mammalian cells and its role in cell proliferation"**
**作者**: Zhang, Y. et al. (2012)
**摘要**: 利用哺乳动物表达系统重组表达了人SURF5蛋白，发现其过表达抑制结肠癌细胞增殖，提示SURF5可能作为肿瘤抑制因子发挥作用。
3. **"Optimization of recombinant human SURF5 production in Pichia pastoris"**
**作者**: Müller, R. et al. (2020)
**摘要**: 报道在毕赤酵母中优化SURF5的重组表达策略，包括密码子优化和纯化方法，获得高产量可溶蛋白，用于后续抗体开发及结构研究。
4. **"SURF5 interacts with mitochondrial ribosomes: evidence from co-immunoprecipitation assays"**
**作者**: Lee, H. et al. (2018)
**摘要**: 通过重组SURF5蛋白与线粒体提取物共孵育，发现其与核糖体蛋白相互作用，提示其可能参与线粒体翻译调控。
**说明**：上述文献为示例性质，实际研究中针对SURF5的功能性文献较少，可能与其在基因簇中的研究相对边缘化有关。建议结合SURF基因家族(如SURF1/SURF2)的研究或利用蛋白质组学数据库(如UniProt)扩展信息。
Surfeit 5 (SURF5), a member of the conserved SURF gene family, is a nuclear-encoded protein primarily localized to the mitochondrial inner membrane in humans. The SURF family comprises six members (SURF1 to SURF6) implicated in diverse cellular processes, though the exact role of SURF5 remains less characterized compared to others like SURF1 (linked to mitochondrial disorders). Structurally, SURF5 is predicted to contain transmembrane domains and a coiled-coil region, suggesting involvement in protein-protein interactions or membrane-associated complexes. Studies hint at its potential role in mRNA processing, mitochondrial ribosome assembly, or oxidative phosphorylation regulation, but mechanistic details are scarce. Notably, SURF5 is ubiquitously expressed across tissues, with higher levels in metabolically active organs like the liver and heart. Recombinant human SURF5 protein (rhSURF5) is typically produced via heterologous expression systems (E. coli, yeast, or mammalian cells) with affinity tags (e.g., His-tag) for purification. Its production facilitates functional studies, including protein interaction mapping, enzymatic activity assays, and mitochondrial dysfunction models. While no direct disease associations are firmly established, SURF5's mitochondrial localization and conserved nature warrant further exploration in metabolic disorders or cancers. Current research gaps include elucidating its molecular partners, precise subcellular roles, and therapeutic relevance.
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