| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SYN3 |
| Uniprot No | O14994 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-580 aa |
| 活性数据 | MNFLRRRLSD SSFMANLPNG YMTDLQRPDS STSSPASPAM ERRHPQPLAA SFSSPGSSLF SSLSSAMKQA PQATSGLMEP PGPSTPIVQR PRILLVIDDA HTDWSKYFHG KKVNGEIEIR VEQAEFSELN LAAYVTGGCM VDMQVVRNGT KVVSRSFKPD FILVRQHAYS MALGEDYRSL VIGLQYGGLP AVNSLYSVYN FCSKPWVFSQ LIKIFHSLGP EKFPLVEQTF FPNHKPMVTA PHFPVVVKLG HAHAGMGKIK VENQLDFQDI TSVVAMAKTY ATTEAFIDSK YDIRIQKIGS NYKAYMRTSI SGNWKANTGS AMLEQVAMTE RYRLWVDSCS EMFGGLDICA VKAVHSKDGR DYIIEVMDSS MPLIGEHVEE DRQLMADLVV SKMSQLPMPG GTAPSPLRPW APQIKSAKSP GQAQLGPQLG QPQPRPPPQG GPRQAQSPQP QRSGSPSQQR LSPQGQQPLS PQSGSPQQQR SPGSPQLSRA SSGSSPNQAS KPGATLASQP RPPVQGRSTS QQGEESKKPA PPHPHLNKSQ SLTNSLSTSD TSQRGTPSED EAKAETIRNL RKSFASLFSD |
| 分子量 | 63.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人Synapsin III(SYN3)蛋白的3篇参考文献示例(文献信息为模拟，需实际检索验证)：
1. **"Expression and functional characterization of recombinant human Synapsin III in neuronal differentiation"**
- 作者：Li X et al.
- 摘要：研究通过哺乳动物细胞系统表达重组人SYN3蛋白，证实其参与调控神经突触囊泡的运输和神经元分化过程。
2. **"Synapsin III deficiency alters dopamine release dynamics in Parkinson's disease models"**
- 作者：Garcia-Reitböck P et al.
- 摘要：利用重组SYN3蛋白进行功能回补实验，发现SYN3缺失导致多巴胺能神经元突触传递异常，与帕金森病病理相关。
3. **"Structural analysis of human Synapsin III reveals phosphorylation-dependent interactions"**
- 作者：Vargas KJ et al.
- 摘要：通过重组蛋白技术解析SYN3磷酸化位点，证明其构象变化影响与突触蛋白的结合能力，调控神经可塑性。
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**提示**：实际文献需通过PubMed/Google Scholar以关键词 "recombinant human SYN3/Synapsin III" 检索，部分研究可能需要通过机构权限获取全文。
Synapsin III (SYN3), a member of the synapsin protein family, plays a critical role in regulating synaptic vesicle dynamics and neurotransmitter release in neurons. Primarily expressed in the central nervous system, SYN3 is involved in modulating synaptic plasticity, neuronal development, and the maintenance of presynaptic structures. Unlike other synapsins (SYN1 and SYN2), SYN3 exhibits distinct expression patterns and functional roles, including interactions with cytoskeletal components and involvement in neurodevelopmental processes. Structurally, it contains conserved domains for vesicle binding and phosphorylation sites that regulate its activity in response to neuronal signaling.
Recombinant human SYN3 protein is engineered using molecular cloning techniques, typically expressed in bacterial or mammalian systems to ensure proper folding and post-translational modifications. This recombinant form enables researchers to study SYN3's biochemical properties, interactions with synaptic partners (e.g., actin, tubulin, and other synapsins), and its implications in neurological disorders. Abnormal SYN3 expression has been linked to conditions like Parkinson's disease, schizophrenia, and bipolar disorder, making it a potential therapeutic target. Current research leverages recombinant SYN3 to investigate its role in dopaminergic neurotransmission, axonal transport, and neuroprotection mechanisms, providing insights for developing targeted interventions in synaptic dysfunction-related diseases.
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