| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SWAP70 |
| Uniprot No | Q9UH65 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-585 aa |
| 活性数据 | MGSLKEELLK AIWHAFTALD QDHSGKVSKS QLKVLSHNLC TVLKVPHDPV ALEEHFRDDD EGPVSNQGYM PYLNRFILEK VQDNFDKIEF NRMCWTLCVK KNLTKNPLLI TEEDAFKIWV IFNFLSEDKY PLIIVSEEIE YLLKKLTEAM GGGWQQEQFE HYKINFDDSK NGLSAWELIE LIGNGQFSKG MDRQTVSMAI NEVFNELILD VLKQGYMMKK GHRRKNWTER WFVLKPNIIS YYVSEDLKDK KGDILLDENC CVESLPDKDG KKCLFLVKCF DKTFEISASD KKKKQEWIQA IHSTIHLLKL GSPPPHKEAR QRRKELRKKQ LAEQEELERQ MKELQAANES KQQELEAVRK KLEEAASRAA EEEKKRLQTQ VELQARFSTE LEREKLIRQQ MEEQVAQKSS ELEQYLQRVR ELEDMYLKLQ EALEDERQAR QDEETVRKLQ ARLLEEESSK RAELEKWHLE QQQAIQTTEA EKQELENQRV LKEQALQEAM EQLEQLELER KQALEQYEEV KKKLEMATNK TKSWKDKVAH HEGLIRLIEP GSKNPHLITN WGPAAFTEAE LEEREKNWKE KKTTE |
| 分子量 | 68.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **文献名称**： "SWAP-70 is a guanine nucleotide exchange factor that mediates signalling of membrane ruffling"
**作者**： Shinohara M., et al.
**摘要**：该研究报道了SWAP70作为Rho GTPase的鸟苷酸交换因子(GEF)，通过调控细胞膜皱褶和肌动蛋白重组，在细胞迁移和免疫反应中发挥作用，并利用重组蛋白验证其体外激活Rho家族蛋白的机制。
2. **文献名称**： "Defective IgG2a/2b class switching in SWAP-70-deficient mice"
**作者**： Jahn T., et al.
**摘要**：通过构建SWAP70基因敲除小鼠模型，发现SWAP70蛋白缺失导致B细胞抗体类别转换(特别是IgG2a/2b)缺陷，表明其在B细胞受体信号通路中调控PI3K介导的膜定位与免疫应答功能。
3. **文献名称**： "SWAP70 regulates Src-kinase accessibility and is essential for mast cell chemotaxis"
**作者**： Sivalenka R.R., Jessberger R.
**摘要**：研究发现重组人SWAP70通过其PH结构域与PI3K产物PIP3结合，调控肥大细胞趋化过程中Src激酶的空间分布，揭示其在细胞定向迁移中的支架蛋白作用。
4. **文献名称**： "Structural basis of membrane targeting by the Dock family protein SWAP70"
**作者**： Dong X., et al.
**摘要**：通过X射线晶体学解析SWAP70的DHR2结构域三维结构，阐明其与细胞膜磷脂互作的机制，并证明重组SWAP70通过结合特定磷脂酸调控免疫突触形成。
**Background of SWAP70 Protein**
SWAP70 (Switching B-cell complex 70 kDa protein) is a unique signal transduction molecule involved in immune regulation, first identified in 2000. Structurally, it contains a pleckstrin homology (PH) domain that binds phosphoinositides and a coiled-coil domain facilitating protein interactions, distinguishing it from typical PH domain-containing proteins. SWAP70 functions as a guanine nucleotide exchange factor (GEF) for Rho-family GTPases, linking membrane signaling to cytoskeletal reorganization.
Primarily expressed in immune cells—including B cells, T cells, dendritic cells, and macrophages—SWAP70 regulates cellular processes like migration, phagocytosis, and antigen presentation. It plays a critical role in B-cell receptor (BCR) signaling, influencing antibody production and class-switching. Studies highlight its involvement in Fcγ receptor-mediated phagocytosis in macrophages and integrin activation in lymphocytes.
SWAP70 deficiency in mice models leads to impaired immune responses, such as reduced antibody diversification and defective T-cell-dependent antigen responses. Conversely, its dysregulation is implicated in autoimmune diseases (e.g., lupus) and cancer progression, where aberrant SWAP70 expression correlates with metastasis and poor prognosis.
Recent research explores SWAP70's dual roles in pro-inflammatory and anti-inflammatory pathways, making it a potential therapeutic target. Its interplay with PI3K/Akt and NF-κB pathways underscores its regulatory complexity in immunity and disease. Further studies aim to clarify its mechanistic nuances and translational applications in immunotherapy and precision medicine.
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