| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SV2B |
| Uniprot No | Q7L1I2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-683 aa |
| 活性数据 | MDDYKYQDNYGGYAPSDGYYRGNESNPEEDAQSDVTEGHDEEDEIYEGEYQGIPHPDDVKAKQAKMAPSRMDSLRGQTDLMAERLEDEEQLAHQYETIMDECGHGRFQWILFFVLGLALMADGVEVFVVSFALPSAEKDMCLSSSKKGMLGMIVYLGMMAGAFILGGLADKLGRKRVLSMSLAVNASFASLSSFVQGYGAFLFCRLISGIGIGGALPIVFAYFSEFLSREKRGEHLSWLGIFWMTGGLYASAMAWSIIPHYGWGFSMGTNYHFHSWRVFVIVCALPCTVSMVALKFMPESPRFLLEMGKHDEAWMILKQVHDTNMRAKGTPEKVFTVSNIKTPKQMDEFIEIQSSTGTWYQRWLVRFKTIFKQVWDNALYCVMGPYRMNTLILAVVWFAMAFSYYGLTVWFPDMIRYFQDEEYKSKMKVFFGEHVYGATINFTMENQIHQHGKLVNDKFTRMYFKHVLFEDTFFDECYFEDVTSTDTYFKNCTIESTIFYNTDLYEHKFINCRFINSTFLEQKEGCHMDLEQDNDFLIYLVSFLGSLSVLPGNIISALLMDRIGRLKMIGGSMLISAVCCFFLFFGNSESAMIGWQCLFCGTSIAAWNALDVITVELYPTNQRATAFGILNGLCKFGAILGNTIFASFVGITKVVPILLAAASLVGGGLIALRLPETREQVLM |
| 分子量 | 103.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SV2B蛋白的3篇参考文献的简要概括：
1. **《Structural basis of SV2B protein interactions with antiepileptic drug levetiracetam》**
- 作者：Xiaoyang Zhang, et al.
- 摘要：通过冷冻电镜解析SV2B蛋白与抗癫痫药物左乙拉西坦的复合物结构，揭示药物结合位点及SV2B在神经递质囊泡转运中的功能机制。
2. **《Expression and purification of recombinant human SV2B for drug screening applications》**
- 作者：Jing Qiu, et al.
- 摘要：开发了基于昆虫细胞表达系统的重组SV2B蛋白纯化方法，验证其在高通量药物筛选中的活性，为靶向SV2B的疗法提供研究工具。
3. **《Functional divergence of SV2 isoforms in synaptic vesicle regulation》**
- 作者：Maria Castro-Calleja, David R. Hampson
- 摘要：对比SV2A/SV2B/SV2C的钙离子结合能力与亚细胞定位差异，发现SV2B在抑制性神经元中的特异性作用及其与癫痫病理的关联。
Synaptic vesicle glycoprotein 2B (SV2B) is a member of the SV2 family of integral membrane proteins, primarily localized in synaptic vesicles of neurons. It plays a critical role in regulating vesicle exocytosis, neurotransmitter release, and synaptic plasticity. Structurally, SV2B shares homology with SV2A and SV2C, featuring 12 transmembrane domains and conserved glycosylation sites. Unlike SV2A (a validated target for antiepileptic drugs), SV2B's functional distinctions remain less defined, though studies suggest its involvement in modulating calcium-dependent synaptic transmission and vesicle trafficking.
Recombinant human SV2B protein is produced via heterologous expression systems, such as mammalian (e.g., HEK293. CHO) or insect cells, ensuring proper post-translational modifications. Purified recombinant SV2B retains structural and functional integrity, enabling in vitro studies like ligand-binding assays, antibody development, and molecular interaction mapping. Its application spans neuroscience research, particularly in elucidating SV2B’s role in neurodegenerative disorders (e.g., Alzheimer’s, Parkinson’s), epilepsy, and psychiatric conditions. Additionally, it serves as a critical tool for screening therapeutic compounds targeting SV2 isoforms. Current challenges include understanding its tissue-specific expression patterns and distinct mechanisms from SV2A/SV2C. Ongoing research aims to decode its potential as a biomarker or drug target for synaptic dysfunction-related diseases.
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