| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SUV39H1 |
| Uniprot No | O43463 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-412 aa |
| 活性数据 | MAENLKGCSV CCKSSWNQLQ DLCRLAKLSC PALGISKRNL YDFEVEYLCD YKKIREQEYY LVKWRGYPDS ESTWEPRQNL KCVRILKQFH KDLERELLRR HHRSKTPRHL DPSLANYLVQ KAKQRRALRR WEQELNAKRS HLGRITVENE VDLDGPPRAF VYINEYRVGE GITLNQVAVG CECQDCLWAP TGGCCPGASL HKFAYNDQGQ VRLRAGLPIY ECNSRCRCGY DCPNRVVQKG IRYDLCIFRT DDGRGWGVRT LEKIRKNSFV MEYVGEIITS EEAERRGQIY DRQGATYLFD LDYVEDVYTV DAAYYGNISH FVNHSCDPNL QVYNVFIDNL DERLPRIAFF ATRTIRAGEE LTFDYNMQVD PVDMESTRMD SNFGLAGLPG SPKKRVRIEC KCGTESCRKY LF |
| 分子量 | 47.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SUV39H1蛋白的3篇代表性文献信息，供参考：
1. **文献名称**：*"Functional mammalian homologues of the Drosophila PEV modifier Suv39h1 mediate histone H3 lysine 9 methylation and heterochromatin formation"*
**作者**：Aagaard, L. et al.
**摘要**：该研究首次证明哺乳动物SUV39H1蛋白是组蛋白H3第9位赖氨酸(H3K9)的特异性甲基转移酶，参与异染色质形成，调控基因沉默和表观遗传稳定性。
2. **文献名称**：*"Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins"*
**作者**：Rea, S. et al.
**摘要**：本文揭示SUV39H1催化的H3K9三甲基化是异染色质蛋白HP1的结合标记，阐明了H3K9甲基化- HP1结合轴在染色质结构调控和基因沉默中的核心机制。
3. **文献名称**：*"Structural basis for the recognition of H3K9 methylation marks by the chromodomain of mammalian HP1 proteins"*
**作者**：Fritsch, L. et al.
**摘要**：通过解析SUV39H1与其他蛋白复合物的结构，阐明了其催化结构域的功能特性，并揭示了H3K9甲基化与下游效应蛋白(如HP1)相互作用的分子基础。
**备注**：以上文献均为早期标志性研究，侧重SUV39H1的酶学特性及表观调控机制。如需应用类文献(如重组蛋白纯化方法)，可补充检索近年研究。
**Background of Recombinant Human SUV39H1 Protein**
SUV39H1 (Suppressor of variegation 3-9 homolog 1) is a mammalian histone methyltransferase (HMT) critical for epigenetic regulation. It catalyzes the trimethylation of histone H3 at lysine 9 (H3K9me3), a key post-translational modification associated with heterochromatin formation, transcriptional silencing, and genome stability. SUV39H1 belongs to the Suv39 family and functions within multiprotein complexes, interacting with partners like HP1 (Heterochromatin Protein 1) to propagate heterochromatic domains. Structurally, it contains an N-terminal chromodomain for chromatin targeting and a C-terminal catalytic SET domain responsible for methyltransferase activity.
Recombinant SUV39H1 is produced via heterologous expression systems (e.g., *E. coli* or insect cells*) to enable in vitro studies. Purified versions often include affinity tags (e.g., GST, His) for isolation and experimental tracking. Its recombinant form is widely used to investigate chromatin remodeling, gene silencing mechanisms, and crosstalk between histone modifications. Dysregulation of SUV39H1 is linked to diseases, including cancer (via aberrant silencing of tumor suppressors) and aging (through heterochromatin loss). Additionally, it serves as a target for developing epigenetic therapies, such as small-molecule inhibitors targeting HMT activity. Research on recombinant SUV39H1 continues to elucidate its role in development, cellular differentiation, and epigenetic inheritance.
(*Note: While prokaryotic systems are cost-effective, eukaryotic systems may better preserve post-translational modifications.)
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