| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SURF6 |
| Uniprot No | O75683 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-361 aa |
| 活性数据 | MASLLAKDAY LQSLAKKICS HSAPEQQART RAGKTQGSET AGPPKKKRKK TQKKFRKREE KAAEHKAKSL GEKSPAASGA RRPEAAKEEA AWASSSAGNP ADGLATEPES VFALDVLRQR LHEKIQEARG QGSAKELSPA ALEKRRRRKQ ERDRKKRKRK ELRAKEKARK AEEATEAQEV VEATPEGACT EPREPPGLIF NKVEVSEDEP ASKAQRRKEK RQRVKGNLTP LTGRNYRQLL ERLQARQSRL DELRGQDEGK AQELEAKMKW TNLLYKAEGV KIRDDERLLQ EALKRKEKRR AQRQRRWEKR TAGVVEKMQQ RQDRRRQNLR RKKAARAERR LLRARKKGRI LPQDLERAGL V |
| 分子量 | 41.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SURF6蛋白的虚构参考文献示例(注意：以下内容为模拟生成，非真实文献)：
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1. **文献名称**: *Cloning and Functional Characterization of Human SURF6: Implications for Ribosome Biogenesis*
**作者**: Zhang L, et al.
**摘要**: 该研究首次报道了人SURF6蛋白的重组表达及功能分析。通过在HEK293细胞中表达带有His标签的SURF6.发现其定位于细胞核仁，并参与前核糖体RNA的加工过程。敲低SURF6导致核糖体亚基组装异常，表明其在核糖体生物合成中发挥关键作用。
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2. **文献名称**: *Recombinant SURF6 Protein Interacts with Mitochondrial Proteins in Apoptotic Pathways*
**作者**: Müller S, et al.
**摘要**: 本研究利用大肠杆菌系统表达并纯化了重组人SURF6蛋白，通过Pull-down实验发现其与线粒体膜蛋白BAX存在相互作用。进一步功能实验表明，SURF6过表达可抑制凋亡信号通路，提示其在线粒体相关细胞死亡调控中的潜在角色。
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3. **文献名称**: *Structural Analysis of SURF6 Using Cryo-EM and Its Role in Genetic Disorders*
**作者**: Gupta R, et al.
**摘要**: 研究结合冷冻电镜解析了重组SURF6蛋白的三维结构，揭示了其N端结构域与RNA结合的可能位点。临床样本分析发现SURF6突变与罕见发育迟缓综合征相关，为其病理机制提供了分子基础。
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4. **文献名称**: *Expression and Purification of Human SURF6 in a Baculovirus System: Application in Antibody Development*
**作者**: Park J, et al.
**摘要**: 本文优化了利用杆状病毒-昆虫细胞系统大规模生产重组SURF6蛋白的方案，纯化蛋白免疫小鼠后获得高特异性单克隆抗体，为SURF6的免疫检测及疾病诊断工具开发奠定了基础。
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提示：若需真实文献，建议通过PubMed或Web of Science搜索“SURF6 protein”或“SURF6 recombinant”。
Surfeit 6 (SURF6) is a conserved vertebrate protein encoded by the SURF gene cluster, a unique group of tightly linked housekeeping genes with unknown evolutionary pressure for their physical clustering. As a member of the SURF family, SURF6 is ubiquitously expressed in human tissues and predominantly localizes to the nucleolus, suggesting roles in ribosomal biogenesis or RNA metabolism. While its precise molecular function remains unclear, studies associate SURF6 with cell proliferation, apoptosis regulation, and embryonic development. It interacts with nucleolar proteins involved in rRNA processing and ribosome assembly, implicating potential contributions to translational regulation.
Recombinant human SURF6 protein is typically produced using expression systems like *E. coli* or mammalian cells, enabling functional studies and antibody development. Research focuses on its involvement in diseases, particularly cancer—elevated SURF6 levels correlate with tumor progression in hepatocellular carcinoma and leukemia, possibly through promoting cell cycle acceleration. Conversely, SURF6 mutations are linked to rare developmental disorders, emphasizing its importance in normal cellular physiology. Current investigations aim to delineate its interaction networks, post-translational modifications, and therapeutic potential as a biomarker or drug target. Despite progress, SURF6’s exact mechanisms and broader biological significance remain active areas of exploration.
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