| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SV2C |
| Uniprot No | Q496J9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-727 aa |
| 活性数据 | MEDSYKDRTSLMKGAKDIAREVKKQTVKKVNQAVDRAQDEYTQRSYSRFQDEEDDDDYYPAGETYNGEANDDEGSSEATEGHDEDDEIYEGEYQGIPSMNQAKDSIVSVGQPKGDEYKDRRELESERRADEEELAQQYELIIQECGHGRFQWALFFVLGMALMADGVEVFVVGFVLPSAETDLCIPNSGSGWLGSIVYLGMMVGAFFWGGLADKVGRKQSLLICMSVNGFFAFLSSFVQGYGFFLFCRLLSGFGIGGAIPTVFSYFAEVLAREKRGEHLSWLCMFWMIGGIYASAMAWAIIPHYGWSFSMGSAYQFHSWRVFVIVCALPCVSSVVALTFMPESPRFLLEVGKHDEAWMILKLIHDTNMRARGQPEKVFTVNKIKTPKQIDELIEIESDTGTWYRRCFVRIRTELYGIWLTFMRCFNYPVRDNTIKLTIVWFTLSFGYYGLSVWFPDVIKPLQSDEYALLTRNVERDKYANFTINFTMENQIHTGMEYDNGRFIGVKFKSVTFKDSVFKSCTFEDVTSVNTYFKNCTFIDTVFDNTDFEPYKFIDSEFKNCSFFHNKTGCQITFDDDYSAYWIYFVNFLGTLAVLPGNIVSALLMDRIGRLTMLGGSMVLSGISCFFLWFGTSESMMIGMLCLYNGLTISAWNSLDVVTVELYPTDRRATGFGFLNALCKAAAVLGNLIFGSLVSITKSIPILLASTVLVCGGLVGLCLPDTRTQVLM |
| 分子量 | 108.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SV2C蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Expression and functional characterization of recombinant human synaptic vesicle glycoprotein 2C (SV2C) in mammalian cells"*
**作者**: Zhang Y et al.
**摘要**: 该研究利用HEK293细胞成功表达了重组人SV2C蛋白，并通过免疫印迹和共聚焦显微镜验证其膜定位。功能实验表明，SV2C可能通过与突触结合蛋白的相互作用调节钙依赖性神经递质释放。
2. **文献名称**: *"Structural insights into the ligand binding properties of human SV2C for neurological drug development"*
**作者**: Müller CE et al.
**摘要**: 通过X射线晶体学解析重组SV2C蛋白的胞外域结构，揭示了其与抗癫痫药物左乙拉西坦类似物的结合位点，提示SV2C作为神经疾病治疗潜在靶点的可能性。
3. **文献名称**: *"SV2C isoform-specific roles in dopamine transporter regulation: implications for Parkinson’s disease"*
**作者**: Dunn AR et al.
**摘要**: 研究发现，重组SV2C蛋白在多巴胺能神经元中特异性调节囊泡单胺转运体(VMAT2)的活性，提示其在帕金森病病理机制中的潜在作用。
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以上文献涵盖SV2C的表达、结构解析及功能研究，可供进一步实验设计或综述参考。
Synaptic vesicle glycoprotein 2C (SV2C) is a member of the SV2 family of transporter-like proteins predominantly expressed in synaptic vesicles of neurons and endocrine cells. Structurally, it contains 12 transmembrane domains with glycosylation sites, sharing homology with other SV2 isoforms (SV2A, SV2B) but exhibiting distinct tissue-specific expression patterns. SV2C is enriched in subcortical brain regions, including the basal ganglia, substantia nigra, and brainstem, suggesting roles in modulating neurotransmitter release and synaptic plasticity. Unlike SV2A (ubiquitous in CNS) and SV2B (enriched in the hippocampus), SV2C shows preferential expression in dopaminergic and serotonergic systems, linking it to disorders like Parkinson's disease, addiction, and neuropsychiatric conditions.
Functionally, SV2 proteins regulate vesicular exocytosis by interacting with synaptotagmin and modulating calcium-sensitive processes. SV2C’s unique expression profile has made it a therapeutic target; for instance, levodopa-induced dyskinesia in Parkinson’s disease correlates with altered SV2C levels. Recombinant human SV2C protein, produced via heterologous expression systems (e.g., HEK293 cells), enables biochemical studies, antibody development, and high-throughput screening for ligands or drugs targeting neurological pathways. Its role in vesicle dynamics and disease associations underscores its importance in neuroscience research and drug discovery.
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