| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SVOP |
| Uniprot No | Q8N4V2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-548 aa |
| 活性数据 | MEEDLFQLRQLPVVKFRRTGESARSEDDTASGEHEVQIEGVHVGLEAVELDDGAAVPKEFANPTDDTFMVEDAVEAIGFGKFQWKLSVLTGLAWMADAMEMMILSILAPQLHCEWRLPSWQVALLTSVVFVGMMSSSTLWGNISDQYGRKTGLKISVLWTLYYGILSAFAPVYSWILVLRGLVGFGIGGVPQSVTLYAEFLPMKARAKCILLIEVFWAIGTVFEVVLAVFVMPSLGWRWLLILSAVPLLLFAVLCFWLPESARYDVLSGNQEKAIATLKRIATENGAPMPLGKLIISRQEDRGKMRDLFTPHFRWTTLLLWFIWFSNAFSYYGLVLLTTELFQAGDVCGISSRKKAVEAKCSLACEYLSEEDYMDLLWTTLSEFPGVLVTLWIIDRLGRKKTMALCFVIFSFCSLLLFICVGRNVLTLLLFIARAFISGGFQAAYVYTPEVYPTATRALGLGTCSGMARVGALITPFIAQVMLESSVYLTLAVYSGCCLLAALASCFLPIETKGRGLQESSHREWGQEMVGRGMHGAGVTRSNSGSQE |
| 分子量 | 87.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SVOP蛋白的3篇代表性文献示例(基于领域内研究方向的合理推测)：
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1. **文献名称**：*Structural characterization of human SVOP reveals its role in synaptic vesicle clustering*
**作者**：Li, X., et al.
**摘要**：通过X射线晶体学解析了重组人SVOP蛋白的晶体结构，揭示了其N端螺旋结构域介导的寡聚化机制，并证明SVOP通过与突触小泡膜蛋白相互作用参与小泡聚集，为神经递质释放机制提供结构基础。
2. **文献名称**：*Recombinant SVOP expression in mammalian cells: A tool for functional neuroscience studies*
**作者**：Zhang, Y., & Wang, H.
**摘要**：开发了一种基于HEK293细胞的重组人SVOP高效表达与纯化方案，证明其在神经元细胞中异源表达后可挽救SVOP缺失导致的突触传递缺陷，为体外研究SVOP功能提供了可靠工具。
3. **文献名称**：*SVOP variants alter protein trafficking and associate with neurodevelopmental disorders*
**作者**：Thompson, R., et al.
**摘要**：通过重组SVOP突变体表达，发现特定错义突变(如p.R207Q)会阻碍其与Rab GTPases的结合，导致小泡运输异常，提示SVOP功能异常可能与自闭症谱系障碍相关。
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**注意**：以上文献为基于领域知识的示例，实际研究中建议通过PubMed或Google Scholar以“SVOP protein recombinant”或“SVOP synaptic vesicle”等关键词检索最新论文。SVOP相关研究尚处于发展阶段，部分方向仍需深入探索。
**Background of SVOP (Secretory Vesicle-associated Membrane Protein):**
SVOP is a transmembrane protein primarily associated with secretory vesicles and lysosome-related organelles in eukaryotic cells. Initially identified in neurons, it plays a critical role in regulating vesicle trafficking, neurotransmitter release, and synaptic function. Structurally, SVOP belongs to the major facilitator superfamily (MFS), characterized by 12 transmembrane domains that facilitate substrate transport across lipid bilayers.
Studies suggest SVOP contributes to maintaining vesicle acidity and ion homeostasis, essential for proper vesicle maturation and cargo sorting. It interacts with key synaptic proteins, including synaptotagmin and V-ATPase, implicating its involvement in neuronal signaling and synaptic plasticity. Beyond the nervous system, SVOP is expressed in secretory tissues (e.g., endocrine cells) and may influence hormone secretion.
Altered SVOP expression or function has been linked to neurological disorders, such as Alzheimer’s disease and schizophrenia, as well as lysosomal storage disorders. Recent research also explores its potential role in cancer cell exosome release and metabolic regulation. Despite its emerging significance, SVOP’s precise molecular mechanisms and substrate specificity remain under investigation, highlighting its importance as a target for understanding vesicle dynamics and disease pathology.
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