| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SYNE1 |
| Uniprot No | Q8NF91 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1561-1670 aa |
| 活性数据 | LRKIQQSVSEFEDKLAVPIKICSSATETYKVLQEHMDLCQALESLSSAITAFSASARKVVNRDSCVQEAAALQQQYEDILRRAKERQTALENLLAHWQRLEKELSSFLTW |
| 分子量 | 37.84kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SYNE1蛋白的3篇参考文献示例(基于公开领域研究方向模拟，非真实文献)：
1. **"Structural Analysis of SYNE1 Domains in Nuclear Envelope Anchoring"**
- **作者**: Zhang Q, et al.
- **摘要**: 研究利用重组SYNE1蛋白片段解析其与核膜相关蛋白(如Emerin)的相互作用，揭示KASH结构域在维持细胞核骨架定位中的关键作用。
2. **"SYNE1 Mutations Disrupt Actin Cytoskeleton Remodeling in Neurons"**
- **作者**: Rajgor D, et al.
- **摘要**: 通过体外重组SYNE1蛋白表达，发现其突变导致神经元细胞骨架重组异常，并与小脑共济失调的病理机制相关联。
3. **"Functional Characterization of SYNE1 Isoforms in Muscular Dystrophy"**
- **作者**: Duong NT, et al.
- **摘要**: 利用重组SYNE1不同剪接变体，证明特定异构体缺失会导致肌肉细胞核极性紊乱，与肌营养不良症相关表型相关。
4. **"SYNE1 Interactions with Microtubule Networks in Cell Migration"**
- **作者**: Misu S, et al.
- **摘要**: 通过重组蛋白共沉淀实验，揭示SYNE1与微管结合蛋白的互作网络，阐明其在细胞迁移及癌症转移中的潜在作用。
(注：以上为模拟文献，实际引用时建议通过PubMed或Google Scholar检索最新真实研究。)
SYNE1 (Spectrin Repeat Containing Nuclear Envelope Protein 1), also known as Nesprin-1. is a giant multi-domain protein encoded by the *SYNE1* gene in humans. It belongs to the nesprin family of proteins that anchor the nucleus to the cytoskeleton by connecting the nuclear envelope to cytoplasmic actin filaments. SYNE1 is a key structural component of the linker of nucleoskeleton and cytoskeleton (LINC) complex, critical for maintaining nuclear integrity, mechanical stability, and cellular positioning during processes like cell migration and mitosis.
The protein contains spectrin repeats, a transmembrane domain, and a conserved KASH (Klarsicht/ANC-1/Syne homology) domain at its C-terminus, enabling interactions with SUN-domain proteins in the nuclear envelope. Mutations in *SYNE1* are linked to various human disorders, including Emery-Dreifuss muscular dystrophy, autosomal recessive cerebellar ataxia, and other neuromuscular pathologies, underscoring its role in tissue-specific mechanical resilience.
Recombinant human SYNE1 protein, produced via heterologous expression systems (e.g., bacteria, mammalian cells), is widely used to study its molecular interactions, nuclear-cytoskeletal coupling mechanisms, and disease-related dysfunction. Its applications span structural biology, mechanotransduction studies, and therapeutic target validation for neuromuscular and neurodegenerative conditions. Research on SYNE1 also contributes to understanding nuclear envelope dynamics in cellular aging and genome organization.
×
