| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAS2R47 |
| Uniprot No | P59541 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-319 aa |
| 活性数据 | MITFLPIIFSILIVVIFVIGNFANGFIALVNSIEWVKRQKISFVDQILTALAVSRVGLLWVLLLHWYATQLNPAFYSVEVRITAYNVWAVTNHFSSWLATSLSMFYLLRIANFSNLIFLRIKRRVKSVVLVILLGPLLFLVCHLFVINMDETVWTKEYEGNVTWKIKLRSAMYHSNMTLTMLANFVPLTLTLISFLLLICSLCKHLKKMQLHGKGSQDPSTKVHIKALQTVTSFLLLCAIYFLSMIISVCNFGRLEKQPVFMFCQAIIFSYPSTHPFILILGNKKLKQIFLSVLRHVRYWVKDRSLRLHRFTRGALCVF |
| 分子量 | 61.49 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于重组人TAS2R47蛋白的研究目前较为有限，可能存在编号误写或该受体尚未被广泛研究。以下提供几篇与人类苦味受体(TAS2R家族)相关的重组蛋白文献供参考，并附上可能的修正建议：
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1. **文献名称**: *"Structural basis for strychnine activation of human bitter taste receptor TAS2R46"*
**作者**: Xu, W. et al.
**摘要**: 通过冷冻电镜解析了人苦味受体TAS2R46与马钱子碱结合的结构，揭示其激活机制，利用重组蛋白技术实现了受体的功能性表达。
2. **文献名称**: *"The molecular basis of individual differences in phenylthiocarbamide perception"*
**作者**: Kim, U. et al.
**摘要**: 研究TAS2R38受体的基因多态性如何影响人类对苯硫脲(PTC)的苦味感知，通过重组蛋白表达验证不同变异体的功能差异。
3. **文献名称**: *"Broad tuning of the human bitter taste receptor hTAS2R46 to various sesquiterpene lactones, clerodane and labdane diterpenoids, and strychnine"*
**作者**: Behrens, M. et al.
**摘要**: 利用重组表达的hTAS2R46受体，系统分析其对多种苦味化合物的反应，揭示其配体多样性。
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**建议**：
- TAS2R47可能为笔误或未被充分研究，常见受体如**TAS2R46**、**TAS2R38**等研究较多。
- 可查阅UniProt或NCBI数据库确认基因编号，或关注近期结构生物学研究(如Nature或Science中关于TAS2R的冷冻电镜成果)。
如需更精准的文献，请提供更多背景信息或确认受体编号准确性。
**Background of Recombinant Human TAS2R47 Protein**
The TAS2R47 protein, a member of the TAS2R (taste 2 receptor) family, is a G protein-coupled receptor (GPCR) primarily associated with bitter taste perception in humans. TAS2R receptors are expressed in taste receptor cells on the tongue and play a critical role in detecting bitter compounds, which often signal potential toxins. TAS2R47. encoded by the *TAS2R47* gene, is characterized by its seven-transmembrane domain structure typical of GPCRs, with extracellular N-terminal and intracellular C-terminal regions. Unlike other TAS2Rs, its ligand specificity remains partially unresolved, though it is hypothesized to bind specific bitter molecules due to structural motifs in its binding pocket.
Recombinant TAS2R47 is produced using heterologous expression systems (e.g., HEK293 cells) for functional studies, enabling exploration of its activation mechanisms and downstream signaling. Upon ligand binding, TAS2R47 couples with gustducin, a G-protein, triggering intracellular calcium release and neurotransmitter signaling to the brain. Research on recombinant TAS2R47 aids in deciphering bitter taste coding, dietary preferences, and potential applications in food science or pharmacology, such as designing bitterness modulators to improve drug palatability. Additionally, its expression in extraoral tissues (e.g., gut, respiratory tract) suggests roles in nutrient sensing or immune responses, broadening its biomedical relevance.
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