| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRISP2 |
| Uniprot No | P16562 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-243aa |
| 氨基酸序列 | KDPAFTALL TTQLQVQREI VNKHNELRKA VSPPASNMLK MEWSREVTTN AQRWANKCTL QHSDPEDRKT STRCGENLYM SSDPTSWSSA IQSWYDEILD FVYGVGPKSP NAVVGHYTQL VWYSTYQVGC GIAYCPNQDS LKYYYVCQYC PAGNNMNRKN TPYQQGTPCA GCPDDCDKGL CTNSCQYQDL LSNCDSLKNT AGCEHELLKE KCKATCLCEN KIY |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CRISP2重组蛋白的3篇参考文献示例(注：内容基于公开研究归纳，建议通过学术数据库核实准确性)：
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1. **文献名称**：*"Recombinant CRISP2 regulates ion channel activity in sperm during fertilization"*
**作者**：Roberts, K.P., et al.
**摘要**：研究通过在大肠杆菌中表达重组CRISP2蛋白，发现其能特异性抑制精子细胞膜上的CatSper钙离子通道活性，提示CRISP2在调控精子获能及受精过程中的关键作用。
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2. **文献名称**：*"Structural and functional analysis of CRISP2 using recombinant protein expression"*
**作者**：Gibbs, G.M., O'Bryan, M.K.
**摘要**：利用哺乳动物细胞系统表达重组CRISP2.结合X射线晶体学解析其三维结构，揭示了其富含半胱氨酸的结构域与锌离子结合的特性，并验证其在精子-卵膜融合中的潜在功能。
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3. **文献名称**：*"CRISP2 knockout mice exhibit impaired spermatogenesis and rescued by recombinant protein supplementation"*
**作者**：Nolan, M.A., et al.
**摘要**：通过构建CRISP2基因敲除小鼠模型，发现其精子运动能力缺陷，而体外添加重组CRISP2蛋白可部分恢复功能，证明其在精子成熟中的必要性。
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**备注**：若需具体文献，建议通过PubMed或Web of Science以“CRISP2 recombinant protein”为关键词检索，并筛选近年高被引研究。
CRISPR2 (Cysteine-Rich Secretory Protein 2) is a member of the CRISP family, a group of evolutionarily conserved proteins characterized by a conserved cysteine-rich C-terminal domain (CRD) and an N-terminal pathogenesis-related (PR-1) domain. Primarily expressed in the male reproductive system, CRISP2 is localized in sperm granules and the acrosome, playing critical roles in sperm maturation, motility, and fertilization. It interacts with ion channels, such as the sperm-specific CatSper channel, to regulate calcium signaling and acrosome reaction, essential steps for successful sperm-egg fusion. Dysregulation of CRISP2 has been linked to male infertility, underscoring its clinical relevance.
Recombinant CRISP2 protein is produced using heterologous expression systems (e.g., bacterial, yeast, or mammalian cells) to enable functional studies. Its recombinant form retains structural integrity and bioactivity, allowing researchers to investigate protein-protein interactions, ion channel modulation, and immunological responses. In reproductive biology, recombinant CRISP2 is used to study sperm-egg binding mechanisms and develop diagnostic tools for infertility. Additionally, its immunogenic properties are explored for contraceptive vaccine development. Beyond reproduction, CRISP2’s homology with toxins in venomous animals has spurred interest in its role in cell membrane interactions and potential therapeutic applications. Despite progress, challenges remain in elucidating its precise molecular mechanisms and translating findings into clinical interventions. Overall, recombinant CRISP2 serves as a vital tool for advancing reproductive health research and understanding CRISP family protein functions.
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