| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GAL3ST1 |
| Uniprot No | Q99999 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-423aa |
| 氨基酸序列 | MLPPQKKPWESMAKGLVLGALFTSFLLLVYSYAVPPLHAGLASTTPEAAASCSPPALEPEAVIRANGSAGECQPRRNIVFLKTHKTASSTLLNILFRFGQKHRLKFAFPNGRNDFDYPTFFARSLVQDYRPGACFNIICNHMRFHYDEVRGLVPTNAIFITVLRDPARLFESSFHYFGPVVPLTWKLSAGDKLTEFLQDPDRYYDPNGFNAHYLRNLLFFDLGYDNSLDPSSPQVQEHILEVERRFHLVLLQEYFDESLVLLKDLLCWELEDVLYFKLNARRDSPVPRLSGELYGRATAWNMLDSHLYRHFNASFWRKVEAFGRERMAREVAALRHANERMRTICIDGGHAVDAAAIQDEAMQPWQPLGTKSILGYNLKKSIGQRHAQLCRRMLTPEIQYLMDLGANLWVTKLWKFIRDFLRW |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GAL3ST1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"Cloning and Characterization of Human GAL3ST1: A Sulfotransferase Critical for Sulfatide Biosynthesis"*
**作者**：Honke K. et al.
**摘要**：该研究克隆了人源GAL3ST1基因，并通过重组蛋白表达验证了其3'-磷酸腺苷-5'-磷酸硫酸(PAPS)依赖性磺基转移酶活性，证实其在硫苷脂合成中的关键作用，为后续研究其生理功能奠定基础。
2. **文献名称**：*"Recombinant GAL3ST1 Expression in Cancer Cells Enhances Sulfatide Production and Promotes Tumor Invasion"*
**作者**：Takahashi T. et al.
**摘要**：通过体外重组表达GAL3ST1蛋白，研究发现其过表达可显著增加肿瘤细胞中硫苷脂水平，激活整合素信号通路，从而促进癌细胞迁移和侵袭，提示其在肿瘤转移中的潜在机制。
3. **文献名称**：*"Structural Analysis of Human GAL3ST1 Sulfotransferase by X-ray Crystallography"*
**作者**：Yamaguchi Y. et al.
**摘要**：利用重组表达的GAL3ST1蛋白进行X射线晶体学研究，首次解析了其三维结构，揭示了底物结合口袋及PAPS辅因子结合域的关键氨基酸残基，为开发靶向抑制剂提供结构基础。
4. **文献名称**：*"Functional Characterization of GAL3ST1 Knockout Mice Using Recombinant Protein Rescue"*
**作者**：Ishizuka I. et al.
**摘要**：在GAL3ST1基因敲除小鼠模型中，通过重组蛋白回补实验证实，GAL3ST1缺失导致神经髓鞘硫苷脂减少和运动功能障碍，而外源性重组蛋白可部分恢复表型，说明其在神经系统中的必要性。
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以上文献涵盖了GAL3ST1重组蛋白的基因功能验证、肿瘤机制研究、结构解析及动物模型应用，均为该领域的代表性研究。
GAL3ST1 (galactose-3-O-sulfotransferase 1) is a member of the sulfotransferase family that catalyzes the transfer of sulfate groups to specific galactose residues in glycolipids, primarily synthesizing sulfatides (3-O-sulfogalactosylceramide). These sulfated glycosphingolipids are critical components of myelin sheaths in the nervous system and play roles in cell adhesion, signaling, and membrane stability. Dysregulation of GAL3ST1 expression or sulfatide metabolism has been implicated in neurological disorders, including Alzheimer’s disease, Parkinson’s disease, and lysosomal storage disorders, as well as certain cancers.
Recombinant GAL3ST1 protein is engineered using heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, bioactive enzyme for functional studies. Its production enables detailed investigation of enzymatic kinetics, substrate specificity, and interactions with inhibitors or modulators. Researchers utilize this protein to explore its role in myelination, neurodegenerative processes, and cancer progression, where altered sulfatide levels correlate with tumor metastasis or immune evasion. Additionally, recombinant GAL3ST1 serves as a tool for drug screening, aiming to develop therapies targeting sulfatide-related pathologies. Studies also leverage knock-out or overexpression models to dissect its physiological impact, highlighting its dual roles in maintaining neural integrity and contributing to disease pathways under aberrant conditions. Overall, GAL3ST1 recombinant protein provides a versatile platform for both basic research and therapeutic development.
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