| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBC1D5 |
| Uniprot No | Q92609 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-795 aa |
| 活性数据 | MYHSLSETRH PLQPEEQEVG IDPLSSYSNK SGGDSNKNGR RTSSTLDSEG TFNSYRKEWE ELFVNNNYLA TIRQKGINGQ LRSSRFRSIC WKLFLCVLPQ DKSQWISRIE ELRAWYSNIK EIHITNPRKV VGQQDLMINN PLSQDEGSLW NKFFQDKELR SMIEQDVKRT FPEMQFFQQE NVRKILTDVL FCYARENEQL LYKQGMHELL APIVFVLHCD HQAFLHASES AQPSEEMKTV LNPEYLEHDA YAVFSQLMET AEPWFSTFEH DGQKGKETLM TPIPFARPQD LGPTIAIVTK VNQIQDHLLK KHDIELYMHL NRLEIAPQIY GLRWVRLLFG REFPLQDLLV VWDALFADGL SLGLVDYIFV AMLLYIRDAL ISSNYQTCLG LLMHYPFIGD VHSLILKALF LRDPKRNPRP VTYQFHPNLD YYKARGADLM NKSRTNAKGA PLNINKVSNS LINFGRKLIS PAMAPGSAGG PVPGGNSSSS SSVVIPTRTS AEAPSHHLQQ QQQQQRLMKS ESMPVQLNKG LSSKNISSSP SVESLPGGRE FTGSPPSSAT KKDSFFSNIS RSRSHSKTMG RKESEEELEA QISFLQGQLN DLDAMCKYCA KVMDTHLVNI QDVILQENLE KEDQILVSLA GLKQIKDILK GSLRFNQSQL EAEENEQITI ADNHYCSSGQ GQGRGQGQSV QMSGAIKQAS SETPGCTDRG NSDDFILISK DDDGSSARGS FSGQAQPLRT LRSTSGKSQA PVCSPLVFSD PLMGPASASS SNPSSSPDDD SSKDSGFTIV SPLDI |
| 分子量 | 89.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **Title**: "TBC1D5 controls the GTPase cycle of Rab7b"
**Authors**: Frasa MA, Maximiano FC, Smolarczyk K, et al.
**Summary**: 研究揭示了TBC1D5作为Rab7b的GTP酶激活蛋白(GAP)，调控其GTP/GDP循环，参与溶酶体定位和自噬体成熟，缺失TBC1D5导致内体-溶酶体运输障碍。
2. **Title**: "VPS35 and TBC1D5 control retrograde transport of amyloid precursor protein"
**Authors**: Kim S, Wairkar YP, Daniels RW, et al.
**Summary**: 发现TBC1D5与VPS35复合物互作，调控逆运输途径中的Rab7活性，影响APP蛋白的运输，可能与阿尔茨海默病的病理进程相关。
3. **Title**: "TBC1D5 links the LC3 conjugation machinery to the Rab GTPase network"
**Authors**: Popovic D, Dikic I.
**Summary**: 阐明TBC1D5通过结合LC3蛋白和抑制Rab7活性，介导自噬体与内体/溶酶体的融合，为自噬质量控制提供分子机制。
4. **Title**: "Structural basis for Rab7b/TPC1D5 complex in lysosomal positioning"
**Authors**: Jia D, Zhang JS, Li F, et al.
**Summary**: 通过结构解析，揭示TBC1D5与Rab7b的相互作用界面，证明其通过调控溶酶体动力蛋白锚定，影响细胞中溶酶体分布与功能。
TBC1D5 is a member of the TBC (Tre-2/Bub2/Cdc16) domain-containing protein family, which functions as GTPase-activating proteins (GAPs) for Rab GTPases, critical regulators of intracellular membrane trafficking. Encoded by the TBC1D5 gene in humans, this protein contains a conserved TBC domain that facilitates its interaction with Rab7a, a key Rab GTPase involved in endosomal sorting, autophagosome-lysosome fusion, and lysosomal degradation. TBC1D5 acts as a Rab7a-specific GAP, promoting the hydrolysis of Rab7a-bound GTP to GDP, thereby modulating Rab7a activity in autophagy and endolysosomal trafficking pathways.
Recombinant human TBC1D5 protein is typically expressed in eukaryotic systems (e.g., HEK293 or insect cells) to ensure proper post-translational modifications and functional integrity. It serves as a vital tool for studying Rab7a regulation, membrane trafficking mechanisms, and autophagic flux in vitro. Researchers utilize purified TBC1D5 to analyze its GAP activity through GTPase assays, map binding interfaces with Rab7a via pull-down experiments, and investigate its role in pathological conditions like neurodegenerative diseases and cancer, where dysfunctional autophagy-lysosome pathways are implicated. Structural studies of recombinant TBC1D5 have also provided insights into its autoinhibitory conformation, which is relieved upon binding to retromer complexes during cargo sorting. Its dual role in retromer-mediated recycling and autophagy underscores its significance in maintaining cellular homeostasis.
×
