| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBC1D3B |
| Uniprot No | A6NDS4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-549 aa |
| 活性数据 | MDVVEVAGSW WAQEREDIIM KYEKGHRAGL PEDKGPKPFR SYNNNVDHLG IVHETELPPL TAREAKQIRR EISRKSKWVD MLGDWEKYKS SRKLIDRAYK GMPMNIRGPM WSVLLNIEEM KLKNPGRYQI MKEKGKRSSE HIQRIDRDIS GTLRKHMFFR DRYGTKQREL LHILLAYEEY NPEVGYCRDL SHIAALFLLY FPEEDAFWAL VQLLASERHS LQGFHSPNGG TVQGLQDQQE HVVATSQSKT MGHQDKKDLC GQCSPLGCLI RILIDGISLG LTLRLWDVYL VEGEQALMPI TRIAFKVQQK RLTKTSRCGP WARFCNRFVD TWARDEDTVL KHLRASMKKL TRKQGDLPPP AKPEQGSSAS RPVPASRGGK TLCKGDRQAP PGPPARFPRP IWSASPPRAP RSSTPCPGGA VREDTYPVGT QGVPSPALAQ GGPQGSWRFL QWNSMPRLPT DLDVEGPWFR HYDFRQSCWV RAISQEDQLA PCWQAEHPAE RVRSAFAAPS TDSDQGTPFR ARDEQQYAPT SGPCLCGLHL ESSQFPPGF |
| 分子量 | 62.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人TBC1D3B蛋白的虚构参考文献(基于领域知识模拟，实际文献需具体检索验证)：
1. **文献名称**：*"TBC1D3B regulates EGFR trafficking and signaling through Rab GTPase activation"*
**作者**：Li, X., Chen, Y., & Wang, J.
**摘要**：本研究报道了重组人TBC1D3B蛋白作为Rab GTP酶激活蛋白(GAP)的活性，证明其通过调控Rab5介导的EGFR内吞作用影响EGFR信号传导。实验通过哺乳动物细胞表达重组TBC1D3B，揭示其功能缺陷导致EGFR降解延迟，促进肿瘤细胞增殖。
2. **文献名称**：*"Recombinant expression and functional characterization of TBC1D3B in breast cancer progression"*
**作者**：Zhang, R., Liu, H., & Wei, F.
**摘要**：通过在大肠杆菌系统中表达并纯化重组TBC1D3B蛋白，结合细胞实验发现其在乳腺癌细胞中高表达可激活MAPK通路，增强细胞迁移和侵袭能力，提示其作为潜在治疗靶点。
3. **文献名称**：*"Structural insights into the TBC1D3B-Rab11A interaction for vesicle trafficking regulation"*
**作者**：Park, S., Kim, M., & Lee, G.
**摘要**：利用重组TBC1D3B蛋白的晶体结构分析，发现其TBC结构域与Rab11A特异性结合，通过冷冻电镜技术阐明其介导内体运输的分子机制，为相关疾病机制提供结构基础。
**说明**：以上内容基于领域知识模拟，实际文献需以数据库(如PubMed、Web of Science)检索结果为准，建议通过关键词“TBC1D3B”、“recombinant”、“Rab GAP”进一步查询近期研究。
TBC1D3B, a member of the TBC1D3 gene family, is a primate-specific protein implicated in cellular signaling and membrane trafficking. This protein contains a conserved TBC (Tre-2/Bub2/Cdc16) domain, which typically functions as a GTPase-activating protein (GAP) for Rab GTPases, regulators of vesicle trafficking. Unique to primates, TBC1D3B arose through gene duplication and positive selection during evolution, suggesting a role in human-specific physiological adaptations. It is frequently amplified or overexpressed in cancers, particularly prostate and breast tumors, where it promotes cell proliferation, migration, and resistance to apoptosis. Mechanistically, TBC1D3B interacts with signaling components like AKT and RAF1. modulating MAPK/ERK and PI3K/AKT pathways to enhance oncogenic signaling. Its C-terminal region harbors multiple phosphorylation sites and protein-binding motifs, enabling dynamic regulation. Recombinant TBC1D3B is produced in vitro to study its structure-function relationships, post-translational modifications, and therapeutic targeting potential. Ongoing research focuses on its dual roles in Rab-mediated trafficking and growth factor signaling, as well as its evolutionary significance in primate development and disease.
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