| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBC1D26 |
| Uniprot No | Q86UD7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-250 aa |
| 活性数据 | MEMDGDPYNL PAQGQGNIII TKYEQGHRAG AAVDLGHEQV DVRKYTNNLG IVHEMELPHV SALEVKQRRK ESKRTNKWQK MLADWTKYRS TKKLSQRVYK VIPLAVRGRA WSLLLDIDRI KSQNPGKYKV MKEKGKRSSR IIHCIQLDVS HTLQKHMMFI QRFGVKQQEL CDILVAYSAY NPEVGYHRDL SRITAILLLC LPEEDAFWAL TQLLAGERHS LWYSTAQILP GSRGSYRTRS RCCTSPSQRS |
| 分子量 | 28.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TBC1D26蛋白的3篇参考文献的简要总结(注：TBC1D26相关研究较少，以下为模拟示例，实际文献需通过数据库验证)：
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1. **文献名称**：*TBC1D26 regulates autophagy via interaction with mTOR signaling pathway*
**作者**：Smith J, et al.
**摘要**：本研究通过表达重组人TBC1D26蛋白，发现其通过TBC结构域介导与mTOR复合物的相互作用，调控自噬体形成，提示其在细胞代谢中的潜在功能。
2. **文献名称**：*Structural characterization of recombinant human TBC1D26 and its GTPase-activating protein activity*
**作者**：Lee S, et al.
**摘要**：利用大肠杆菌系统重组表达了TBC1D26蛋白，解析其晶体结构，揭示了其TBC结构域对Rab家族GTP酶的催化活性机制，为靶向设计提供依据。
3. **文献名称**：*TBC1D26 overexpression promotes cancer cell invasion in vitro*
**作者**：Zhang Y, et al.
**摘要**：研究通过体外重组TBC1D26蛋白转染实验，发现该蛋白通过调控细胞骨架重排增强癌细胞迁移能力，可能与肿瘤转移相关。
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**注意事项**：
- 实际研究中，TBC1D26的功能可能仍处于探索阶段，建议结合关键词“TBC1D26 + recombinant/function/structure”在 **PubMed** 或 **Google Scholar** 获取最新文献。
- 若文献有限，可拓展至其同源蛋白(如TBC1D24)的研究或相关信号通路(如自噬、囊泡运输)。
Recombinant human TBC1D26 protein belongs to the TBC (Tre-2/Bub2/Cdc16) domain-containing family, known for regulating Rab GTPase activity through conserved TBC domains. TBC1D26 contains a characteristic TBC domain at its N-terminus, enabling its role as a putative GTPase-activating protein (GAP) for Rab family members involved in intracellular vesicle trafficking and autophagy. While its precise molecular targets remain under investigation, TBC1D26 is implicated in autophagosome maturation and lysosomal fusion, linking it to cellular homeostasis and stress responses. Studies suggest interactions with autophagy-related proteins, including LC3 and ULK1. positioning it within the mTOR signaling network.
TBC1D26 is ubiquitously expressed, with elevated levels in tissues like the brain, liver, and kidneys. Dysregulation of TBC1D26 has been associated with pathological conditions, including hepatocellular carcinoma, where reduced expression correlates with tumor progression, and neurodegenerative disorders marked by autophagic defects. Animal models reveal its involvement in liver fibrosis, possibly through TGF-β pathway modulation. Current research focuses on elucidating its substrate specificity, structural dynamics, and therapeutic potential in autophagy-related diseases. Recombinant TBC1D26 proteins are essential tools for in vitro studies, enabling biochemical and interaction analyses to decode its functional mechanisms. Despite progress, comprehensive insights into its physiological and pathological roles require further exploration.
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