| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBC1D20 |
| Uniprot No | Q96BZ9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-403 aa |
| 活性数据 | MALRSAQGDGPTSGHWDGGAEKADFNAKRKKKVAEIHQALNSDPTDVAALRRMAISEGGLLTDEIRRKVWPKLLNVNANDPPPISGKNLRQMSKDYQQVLLDVRRSLRRFPPGMPEEQREGLQEELIDIILLILERNPQLHYYQGYHDIVVTFLLVVGERLATSLVEKLSTHHLRDFMDPTMDNTKHILNYLMPIIDQVNPELHDFMQSAEVGTIFALSWLITWFGHVLSDFRHVVRLYDFFLAYHPLMPIYFAAVIVLYREQEVLDCDCDMASVHHLLSQIPQDLPYETLISRAGDLFVQFPPSELAREAAAQQQAERTAASTFKDFELASAQQRPDMVLRQRFRGLLRPEDRTKDVLTKPRTNRFVKLAVMGLTVALGAAALAVVKSALEWAPKFQLQLFP |
| 分子量 | 72.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TBC1D20蛋白的3篇代表性文献及其摘要概览：
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1. **文献名称**：*TBC1D20 deficiency causes Warburg Micro syndrome via dysregulated Rab GTPase-dependent mitochondrial dynamics*
**作者**：Sidjanin DJ, et al.
**摘要**：本研究揭示了TBC1D20基因突变与Warburg微综合征的关联。TBC1D20通过调控Rab1和Rab2 GTP酶活性，参与线粒体动态平衡和自噬过程，其功能缺陷导致神经发育异常和眼部病变。
2. **文献名称**：*TBC1D20 mediates the interaction of hepatitis C virus NS5A with Rab-GTPases to coordinate viral replication*
**作者**：Sklan EH, et al.
**摘要**：研究发现TBC1D20与丙型肝炎病毒(HCV)的非结构蛋白NS5A直接结合，通过抑制Rab1的GTP酶活性，重塑宿主细胞内膜结构(如内质网)，从而促进病毒复制复合体的形成。
3. **文献名称**：*The TBC1D20-Rab1/2 axis regulates ER-to-Golgi trafficking and autophagy*
**作者**：Neuman SD, et al.
**摘要**：该研究阐明了TBC1D20作为Rab1/Rab2特异性GAP蛋白，在内质网-高尔基体膜转运及自噬调控中的关键作用，其缺失导致分泌途径紊乱和自噬体成熟受阻。
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**说明**：以上文献主题涵盖TBC1D20在疾病(如Warburg微综合征)、病毒复制及细胞运输中的功能机制，核心围绕其对Rab GTP酶的调控作用。实际引用时建议通过PubMed等平台核对具体信息。
**Background of Recombinant Human TBC1D20 Protein**
TBC1D20 (Tre-2/Bub2/Cdc16 domain family member 20) is a conserved eukaryotic protein belonging to the TBC (Tre-2/Bub2/Cdc16) family, characterized by a TBC domain responsible for Rab GTPase-activating protein (GAP) activity. It regulates intracellular vesicle trafficking by inactivating specific Rab GTPases, notably Rab1 and Rab2. which are critical for endoplasmic reticulum (ER)-to-Golgi transport. TBC1D20 localizes to the ER membrane and interacts with lipid droplets, suggesting roles in lipid metabolism and membrane dynamics.
Studies highlight its involvement in viral pathogenesis, particularly hepatitis C virus (HCV) replication, where TBC1D20 is hijacked to facilitate viral assembly by altering lipid droplet morphology. Additionally, TBC1D20 dysfunction has been linked to male infertility in mice due to disrupted spermatogenesis, underscoring its importance in reproductive biology.
Recombinant human TBC1D20 protein is typically produced in expression systems like *E. coli* or mammalian cell lines (e.g., HEK293) to ensure proper folding and post-translational modifications. This recombinant form enables *in vitro* studies of its GAP activity, interactions with viral proteins, and mechanisms in membrane trafficking. Its applications span basic research on cellular transport, antiviral drug development, and understanding molecular bases of reproductive disorders. TBC1D20’s dual roles in fundamental cellular processes and disease pathways make it a compelling target for biomedical research.
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