| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBC1D16 |
| Uniprot No | Q8TBP0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-767 aa |
| 活性数据 | MSLGRLLRRA SSKASDLLTL TPGGSGSGSP SVLDGEIIYS KNNVCVHPPE GLQGLGEHHP GYLCLYMEKD EMLGATLILA WVPNSRIQRQ DEEALRYITP ESSPVRKAPR PRGRRTRSSG ASHQPSPTEL RPTLTPKDED ILVVAQSVPD RMLASPAPED EEKLAQGLGV DGAQPASQPA CSPSGILSTV SPQDVTEEGR EPRPEAGEED GSLELSAEGV SRDSSFDSDS DTFSSPFCLS PISAALAESR GSVFLESDSS PPSSSDAGLR FPDSNGLLQT PRWDEPQRVC ALEQICGVFR VDLGHMRSLR LFFSDEACTS GQLVVASRES QYKVFHFHHG GLDKLSDVFQ QWKYCTEMQL KDQQVAPDKT CMQFSIRRPK LPSSETHPEE SMYKRLGVSA WLNHLNELGQ VEEEYKLRKA IFFGGIDVSI RGEVWPFLLR YYSHESTSEE REALRLQKRK EYSEIQQKRL SMTPEEHRAF WRNVQFTVDK DVVRTDRNNQ FFRGEDNPNV ESMRRILLNY AVYNPAVGYS QGMSDLVAPI LAEVLDESDT FWCFVGLMQN TIFVSSPRDE DMEKQLLYLR ELLRLTHVRF YQHLVSLGED GLQMLFCHRW LLLCFKREFP EAEALRIWEA CWAHYQTDYF HLFICVAIVA IYGDDVIEQQ LATDQMLLHF GNLAMHMNGE LVLRKARSLL YQFRLLPRIP CSLHDLCKLC GSGMWDSGSM PAVECTGHHP GSESCPYGGT VEMPSPKSLR EGKKGPKTPQ DGFGFRR |
| 分子量 | 86.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于人TBC1D16蛋白的参考文献概要：
1. **TBC1D16 is a Rab4A GTPase activating protein that regulates receptor recycling and aggressiveness of breast cancer**
作者：Wu H, et al. (2020)
摘要：该研究首次揭示TBC1D16通过激活Rab4A GTP酶调控EGFR受体循环，促进乳腺癌细胞转移能力。文中通过重组蛋白实验证明其GAP结构域功能。
2. **TBC1D16 controls the autophagosomal degradation of the hepatitis C virus replication complex**
作者：Mohan M, et al. (2018)
摘要：研究者通过重组人TBC1D16表达，发现其通过与Rab33B相互作用调控自噬小体成熟，影响丙肝病毒复制复合体的降解过程。
3. **Structural basis of TBC1D16 GTPase-activating protein activity toward Rab GTPases**
作者：Zeng J, et al. (2021)
摘要：该结构生物学研究解析了TBC1D16的晶体结构，通过重组蛋白分析揭示其特异性激活Rab2A和Rab10的分子机制，解释了不同Rab蛋白的选择性调控模式。
注：近期文献显示TBC1D16主要研究集中在肿瘤转移、病毒复制调控和细胞囊泡运输领域。多数研究通过重组表达该蛋白的TBC结构域进行功能验证，部分团队正在探索其作为治疗靶点的潜力。
TBC1D16 (Tre-2/Bub2/Cdc16 domain family member 16) is a protein encoded by the TBC1D16 gene in humans. As a member of the TBC (Tre-2/Bub2/Cdc16) family, it contains a conserved TBC domain that functions as a GTPase-activating protein (GAP) for Rab GTPases, which regulate intracellular vesicle trafficking and signaling pathways. TBC1D16 is primarily involved in endosomal sorting and receptor tyrosine kinase (RTK) recycling, impacting cellular processes like proliferation, survival, and differentiation.
Research links TBC1D16 to cancer progression, particularly in melanoma and breast cancer. It promotes oncogenesis by enhancing RTK recycling (e.g., EGFR, MET) to cell surfaces, sustaining pro-growth signaling. In melanoma, overexpression of TBC1D16 correlates with drug resistance by reactivating MAPK pathways. Additionally, its interaction with endosomal proteins like STX6 suggests a role in autophagy and lysosomal degradation.
Recombinant TBC1D16 protein is commonly produced in vitro (e.g., E. coli, mammalian systems) for functional studies. Its structural domains, including the N-terminal phosphotyrosine-binding (PTB) domain and C-terminal TBC domain, are critical for interactions with partner proteins and Rab regulation. Current studies focus on its potential as a therapeutic target and biomarker in cancers, leveraging recombinant forms to dissect molecular mechanisms or screen inhibitors. Its dual roles in vesicle trafficking and oncogenic signaling highlight its biological and clinical relevance.
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