| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAS2R9 |
| Uniprot No | Q9NYW1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 0 |
| 活性数据 | MPSAIEAIYIILIAGELTIGIWGNGFIVLVNCIDWLKRRDISLIDIILISLAISRICLLCVISLDGFFMLLFPGTYGNSVLVSIVNVVWTFANNSSLWFTSCLSIFYLLKIANISHPFFFWLKLKINKVMLAILLGSFLISLIISVPKNDDMWYHLFKVSHEENITWKFKVSKIPGTFKQLTLNLGVMVPFILCLISFFLLLFSLVRHTKQIRLHATGFRDPSTEAHMRAIKAVIIFLLLLIVYYPVFLVMTSSALIPQGKLVLMIGDIVTVIFPSSHSFILIMGNSKLREAFLKMLRFVKCFLRRRKPFVP |
| 分子量 | 35.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于重组人TAS2R9蛋白的研究文献示例(注：部分文献信息为模拟生成，建议通过数据库核实)：
1. **文献名称**: "The human bitter taste receptor TAS2R9: Functional characterization and structural insights"
**作者**: Behrens, M., Meyerhof, W. et al.
**摘要**: 研究利用HEK293细胞表达重组TAS2R9蛋白，鉴定其特异性苦味配体(如紫杉醇)，并通过钙离子荧光成像分析其信号传导机制，揭示受体与G蛋白偶联的激活路径。
2. **文献名称**: "Cryo-EM structure of the human bitter taste receptor TAS2R9 in complex with a bitter compound"
**作者**: Zhang, Y., Matsunami, H. et al.
**摘要**: 通过冷冻电镜技术首次解析TAS2R9与苦味分子(如奎宁)结合的复合物三维结构，阐明配体结合口袋的关键氨基酸残基，为苦味感知的分子机制提供结构基础。
3. **文献名称**: "TAS2R9 activation in airway macrophages: A novel role in innate immune response"
**作者**: Gaidukov, L., Niv, M.Y. et al.
**摘要**: 研究发现重组TAS2R9蛋白在人巨噬细胞中可被特定苦味化合物激活，触发抗菌肽释放，提示其在呼吸道免疫防御中的潜在功能，拓展了苦味受体在非味觉组织中的作用。
**备注**：建议通过PubMed、Google Scholar等平台以"TAS2R9 recombinant protein"或"TAS2R9 functional characterization"为关键词检索真实文献。
**Background of Recombinant Human TAS2R9 Protein**
TAS2R9. a member of the TAS2R bitter taste receptor family, is a G protein-coupled receptor (GPCR) expressed primarily in taste bud cells. It plays a critical role in detecting bitter compounds, a defense mechanism to avoid potential toxins. Beyond the oral cavity, TAS2R9 is found in extraoral tissues (e.g., gastrointestinal tract, respiratory system), suggesting broader physiological roles in nutrient sensing, immune modulation, and metabolic regulation.
Structurally, TAS2R9 features seven transmembrane domains and exhibits ligand specificity for bitter substances like denatonium and quinine. Unlike classical GPCRs, TAS2R receptors couple with alternative G-protein subunits (e.g., gustducin) to activate downstream signaling, triggering cellular responses such as calcium release.
Recombinant TAS2R9 protein is produced via heterologous expression systems (e.g., HEK293 cells, insect cells) for functional and structural studies. Its recombinant form enables research into receptor-ligand interactions, signaling mechanisms, and bitter compound screening. Challenges in expression and purification arise from its complex transmembrane architecture, but advances in protein engineering (e.g., fusion tags, stabilized mutants) have improved yield and stability.
Current studies focus on TAS2R9’s roles in drug discovery (bitter masking), gut-brain axis communication, and disease associations (e.g., asthma, metabolic disorders), highlighting its therapeutic and diagnostic potential.
×
