| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAS2R60 |
| Uniprot No | P59551 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-318 aa |
| 活性数据 | MNGDHMVLGSSVTDKKAIILVTILLLLRLVAIAGNGFITAALGVEWVLRRMLLPCDKLLV SLGASRFCLQSVVMGKTIYVFLHPMAFPYNPVLQFLAFQWDFLNAATLWSSTWLSVFYCV KIATFTHPVFFWLKHKLSGWLPWMLFSSVGLSSFTTILFFIGNHRMYQNYLRNHLQPWNV TGDSIRSYCEKFYLFPLKMITWTMPTAVFFICMILLITSLGRHRKKALLTTSGFREPSVQ AHIKALLALLSFAMLFISYFLSLVFSAAGIFPPLDFKFWVWESVIYLCAAVHPIILLFSN CRLRAVLKSRRSSRCGTP |
| 分子量 | 36.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人TAS2R60蛋白的参考文献概览：
1. **"Expression and functional characterization of the bitter taste receptor TAS2R60 in human cells"**
- **作者**: Meyerhof W., et al.
- **摘要**: 研究利用HEK293细胞成功表达重组人TAS2R60蛋白，并通过钙离子成像技术鉴定其特异性地被萜类化合物(如马钱子碱)激活，揭示了该受体在苦味感知中的配体多样性。
2. **"Structural insights into TAS2R60 activation by bitter compounds"**
- **作者**: Zhang Y., et al.
- **摘要**: 通过冷冻电镜解析重组TAS2R60蛋白与马兜铃酸A的复合物结构，揭示了其配体结合口袋的关键氨基酸残基，为苦味受体激活机制提供了分子层面的解释。
3. **"Recombinant TAS2R60 signaling in airway epithelial cells: implications for innate immunity"**
- **作者**: Lee RJ., Cohen NA.
- **摘要**: 发现重组TAS2R60在呼吸道上皮细胞中表达后可被特定苦味物质激活，触发一氧化氮释放及抗菌肽分泌，提示其在黏膜免疫防御中的潜在作用。
注：TAS2R60的研究相对较少，部分文献可能集中于TAS2R家族其他成员(如TAS2R38)，以上示例为假设性内容，实际研究需参考真实数据库(如PubMed)检索结果。
TAS2R60 is a member of the human taste receptor type 2 (TAS2R) family, a group of G protein-coupled receptors (GPCRs) primarily involved in bitter taste perception. Encoded by the TAS2R60 gene, this protein is expressed in taste receptor cells on the tongue, where it detects specific bitter compounds as part of a protective mechanism to avoid potential toxins. Unlike other TAS2R receptors with broad ligand specificity, TAS2R60 is known to respond to select bitter stimuli, though its exact natural ligands remain under investigation. Studies suggest it may play a role in recognizing certain plant-derived alkaloids or synthetic compounds.
Recombinant TAS2R60 protein, produced through heterologous expression systems like mammalian or insect cells, enables detailed structural and functional analyses. Its recombinant form retains ligand-binding capabilities, allowing researchers to study activation mechanisms, receptor-ligand interactions, and downstream signaling pathways involving gustducin (the associated G-protein). This has implications for understanding taste coding and evolutionary adaptations in dietary preferences.
Beyond taste perception, TAS2R60 is found in non-gustatory tissues (e.g., gut, airways), suggesting additional roles in nutrient sensing, gut motility regulation, or innate immune responses. Polymorphisms in TAS2R60 may influence individual sensitivity to bitter compounds, potentially affecting dietary choices or drug compliance. Current research focuses on its therapeutic potential, including bitter taste modulation in nutrition and medicine, as well as its emerging roles in metabolic and respiratory diseases.
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