| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAS2R5 |
| Uniprot No | Q9NYW4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 0 |
| 活性数据 | MLSAGLGLLMLVAVVEFLIGLIGNGSLVVWSFREWIRKFNWSSYNLIILGLAGCRFLLQWLIILDLSLFPLFQSSRWLRYLSIFWVLVSQASLWFATFLSVFYCKKITTFDRPAYLWLKQRAYNLSLWCLLGYFIINLLLTVQIGLTFYHPPQGNSSIRYPFESWQYLYAFQLNSGSYLPLVVFLVSSGMLIVSLYTHHKKMKVHSAGRRDVRAKAHITALKSLGCFLLLHLVYIMASPFSITSKTYPPDLTSVFIWETLMAAYPSLHSLILIMGIPRVKQTCQKILWKAVCARRCWGP |
| 分子量 | 34.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人TAS2R5蛋白的示例参考文献(注：部分文献信息为示例性概括，实际引用需核实原文)：
1. **《Functional characterization of the bitter taste receptor hTAS2R5 in HEK293 cells》**
- 作者: Behrens, M., et al.
- 摘要: 研究通过重组表达系统在HEK293细胞中表达了hTAS2R5.证实其对特定苦味化合物(如马钱子碱)的激活作用，并分析了其下游信号通路与钙离子动员的关联。
2. **《Structural insights into the ligand binding properties of hTAS2R5》**
- 作者: Brockhoff, A., et al.
- 摘要: 利用重组hTAS2R5蛋白的体外表达，结合分子对接实验，揭示了该受体与多种苦味配体的结合位点及特异性，阐明了其构效关系。
3. **《Expression optimization and pharmacological profiling of recombinant hTAS2R5 in insect cells》**
- 作者: Marchal, S., et al.
- 摘要: 描述了在昆虫细胞中高效表达重组hTAS2R5蛋白的优化策略，并通过高通量筛选鉴定了新型苦味激动剂，为苦味受体药物开发提供模型。
4. **《TAS2R5 polymorphisms alter receptor sensitivity to bitter compounds》**
- 作者: Meyerhof, W., et al.
- 摘要: 通过重组TAS2R5蛋白的功能实验，发现特定基因突变(如A49P)显著改变受体对苯硫脲(PTC)等苦味物质的响应阈值，揭示了人群苦味感知差异的分子机制。
(注：以上文献为示例性内容，具体研究请参考真实数据库如PubMed或Web of Science。)
TAS2R5. a member of the human bitter taste receptor family (TAS2Rs), belongs to the G protein-coupled receptor (GPCR) superfamily. It is predominantly expressed in type II taste receptor cells of lingual taste buds, where it detects bitter compounds as part of an evolutionary defense mechanism against toxic substances. TAS2R5 is activated by specific bitter ligands such as denatonium benzoate and aloin, triggering intracellular signaling via gustducin G-proteins. This activates phospholipase Cβ2 (PLCβ2), leading to calcium release and transient receptor potential (TRP) channel activation, ultimately transmitting bitter signals to the brain.
Beyond taste perception, TAS2R5 is found in extra-oral tissues including respiratory, gastrointestinal, and reproductive systems, suggesting roles in innate immunity, inflammation modulation, and metabolic regulation. Its widespread distribution implies broader physiological functions, though mechanisms remain poorly understood.
Recombinant TAS2R5 protein, typically produced in heterologous systems (e.g., HEK293 or insect cells), enables structural and functional studies. Tagged versions (e.g., FLAG/His-tag) facilitate purification and receptor trafficking analysis. Research leverages recombinant TAS2R5 for bitter ligand screening, receptor activation pathway mapping, and drug development targeting bitter signaling pathways. Challenges persist in maintaining native conformation during recombinant expression due to complex GPCR folding requirements. Advances in stable cell lines and detection methods (e.g., calcium imaging, luciferase assays) continue to enhance its study. Understanding TAS2R5 may inform strategies for managing bitter taste-related dietary compliance or therapeutic interventions.
×
