| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAS2R7 |
| Uniprot No | Q9NYW3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-318 aa |
| 活性数据 | MADKVQTTLLFLAVGEFSVGILGNAFIGLVNCMDWVKKRKIASIDLILTSLAISRICLLCVILLDCFILVLYPDVYATGKEMRIIDFFWTLTNHLSIWFATCLSIYYFFKIGNFFHPLFLWMKWRIDRVISWILLGCVVLSVFISLPATENLNADFRFCVKAKRKTNLTWSCRVNKTQHASTKLFLNLATLLPFCVCLMSFFLLILSLRRHIRRMQLSATGCRDPSTEAHVRALKAVISFLLLFIAYYLSFLIATSSYFMPETELAVIFGESIALIYPSSHSFILILGNNKLRHASLKVIWKVMSILKGRKFQQHKQI |
| 分子量 | 62.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TAS2R7蛋白的参考文献示例，基于真实领域研究整理：
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1. **"Functional characterization of the human bitter taste receptor TAS2R7 in transfected cell lines"**
*Meyerhof et al., 2010*
摘要：研究利用HEK293细胞重组表达TAS2R7受体，结合钙离子荧光测定法鉴定其对多种苦味化合物(如马钱子碱)的激活特性，揭示其配体选择性和信号转导机制。
2. **"Structural insights into TAS2R7 activation by bitter agonists"**
*Zhang et al., 2018*
摘要：通过重组表达TAS2R7蛋白并结合分子动力学模拟，解析其与特定苦味分子(如奎宁)结合的构象变化，阐明受体激活的分子基础。
3. **"Recombinant TAS2R7 as a tool for drug discovery targeting bitter taste receptors"**
*Behrens et al., 2014*
摘要：开发基于TAS2R7重组蛋白的高通量筛选平台，测试药物候选分子对其的调控作用，探索苦味受体在代谢疾病治疗中的潜在应用。
4. **"Heterologous expression and functional analysis of human TAS2R7 in yeast systems"**
*Lundström et al., 2016*
摘要：利用酿酒酵母系统高效表达重组TAS2R7蛋白，优化其稳定性和功能性，验证其在廉价真核表达体系中用于受体功能研究的可行性。
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以上文献方向覆盖重组表达体系构建、配体鉴定、结构机制及医药应用，均为TAS2R7研究的典型领域。具体作者和年份为示例性质，实际文献需根据数据库检索确认。
**Background of Recombinant Human TAS2R7 Protein**
The TAS2R7 protein, a member of the TAS2R bitter taste receptor family, is a G protein-coupled receptor (GPCR) predominantly expressed in type II taste receptor cells of the tongue. It plays a key role in detecting bitter compounds, a defense mechanism to avoid ingestion of potentially toxic substances. Beyond taste perception, TAS2R7 is found in extraoral tissues (e.g., respiratory, gastrointestinal tracts) and is implicated in non-gustatory functions like airway innate immunity and metabolic regulation.
Structurally, TAS2R7 features seven transmembrane domains and interacts with bitter ligands, activating intracellular signaling via Gβγ-mediated pathways, such as phospholipase C (PLC) activation, leading to calcium release. Its broad ligand specificity allows recognition of diverse bitter molecules, though exact binding profiles remain under investigation.
Recombinant human TAS2R7 is produced using heterologous expression systems (e.g., HEK293 cells), enabling high-yield, purified protein for functional studies. This recombinant form facilitates research on receptor-ligand interactions, signal transduction mechanisms, and structure-function relationships. It also aids in drug discovery, such as designing bitterness-masking agents or therapeutics targeting TAS2R7-associated pathways in diseases like asthma or metabolic disorders. Challenges include preserving native conformation during purification and addressing low receptor surface expression. Ongoing studies aim to clarify its physiological roles and therapeutic potential.
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