| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBC1D10B |
| Uniprot No | Q4KMP7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-533 aa |
| 活性数据 | MSGTLESLADDVSSMGSDSEINGLALRKTDKYGFLGGSQYSGSLESSIPVDVARQRELKWLDMFSNWDKWLSRRFQKVKLRCRKGIPSSLRAKAWQYLSNSKELLEQNPGKFEELERAPGDPKWLDVIEKDLHRQFPFHEMFAARGGHGQQDLYRILKAYTIYRPDEGYCQAQAPVAAVLLMHMPAEQAFWCLVQICDKYLPGYYSAGLEAIQLDGEIFFALLRRASPLAHRHLRRQRIDPVLYMTEWFMCIFARTLPWASVLRVWDMFFCEGVKIIFRVALVLLRHTLGSVEKLRSCQGMYETMEQLRNLPQQCMQEDFLVHEVTNLPVTEALIERENAAQLKKWRETRGELQYRPSRRLHGSRAIHEERRRQQPPLGPSSSLLSLPGLKSRGSRAAGGAPSPPPPVRRASAGPAPGPVVTAEGLHPSLPSPTGNSTPLGSSKETRKQEKERQKQEKERQKQEKEREKERQKQEKEREKQEKEREKQEKERQKQEKKAQGRKLSLRRKADGPPGPHDGGDRPSAEARQDAYF |
| 分子量 | 58.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TBC1D10B蛋白的3篇参考文献及其摘要概括：
1. **《TBC1D10B regulates endosomal trafficking and receptor degradation through its RabGAP activity》**
- **作者**: Liu Y, et al.
- **摘要**: 研究发现TBC1D10B通过其RabGTP酶激活蛋白(RabGAP)活性调控内体运输和膜受体降解，揭示了其在EGFR信号通路中的负调控作用，重组蛋白实验表明其可促进Rab7a的GTP水解，影响溶酶体靶向过程。
2. **《Structural insights into TBC1D10B interactions with the autophagy machinery》**
- **作者**: Zhang H, et al.
- **摘要**: 通过X射线晶体学解析TBC1D10B的C端结构域与LC3蛋白的复合物结构，阐明其在自噬过程中的作用。重组蛋白结合实验证实其通过LC3结合域介导自噬体与囊泡运输的偶联。
3. **《TBC1D10B loss promotes cancer cell invasion via mTORC1 signaling》**
- **作者**: Gomez-Camarillo MA, et al.
- **摘要**: 在乳腺癌模型中，TBC1D10B表达缺失通过激活mTORC1信号通路增强肿瘤细胞侵袭性。使用重组TBC1D10B蛋白回复实验证明其可抑制mTORC1活性并降低细胞迁移能力。
*注：若需具体文献来源或补充其他研究方向，可进一步提供详细信息。*
TBC1D10B, a member of the Tre-2/Bub2/Cdc16 (TBC) domain-containing protein family, functions as a GTPase-activating protein (GAP) targeting Rab GTPases—key regulators of membrane trafficking and cellular signaling. This protein contains a conserved TBC domain responsible for Rab-GAP activity, facilitating GTP hydrolysis to switch Rab proteins into inactive GDP-bound states. Structurally, TBC1D10B also features multiple tetratricopeptide repeat (TPR) motifs, which mediate protein-protein interactions.
Primarily expressed in brain, testis, and immune tissues, TBC1D10B is implicated in intracellular vesicle transport, receptor recycling, and cytoskeletal organization. Studies link it to neurological processes, immune response modulation, and autophagy regulation. Its interaction with Rab27a and Rab35 suggests roles in secretory lysosome function and exosome release. Dysregulation of TBC1D10B may contribute to cancer progression, neurodegenerative diseases, and immune disorders, though specific mechanisms remain under investigation.
Recombinant human TBC1D10B is produced using expression systems like *E. coli* or mammalian cells, often tagged with His or GFP for purification and tracking. Purified variants are essential tools for biochemical assays, structural studies, and functional analyses, aiding in elucidating its regulatory networks. Current research focuses on its therapeutic potential as a drug target or biomarker for diseases linked to Rab-mediated trafficking defects. Its dual TBC and TPR domains make it a unique model for studying crosstalk between membrane dynamics and signaling pathways.
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