| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBC1D10C |
| Uniprot No | Q8IV04 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-446 aa |
| 活性数据 | MAQALGEDLV QPPELQDDSS SLGSDSELSG PGPYRQADRY GFIGGSSAEP GPGHPPADLI RQREMKWVEM TSHWEKTMSR RYKKVKMQCR KGIPSALRAR CWPLLCGAHV CQKNSPGTYQ ELAEAPGDPQ WMETIGRDLH RQFPLHEMFV SPQGHGQQGL LQVLKAYTLY RPEQGYCQAQ GPVAAVLLMH LPPEEAFWCL VQICEVYLPG YYGPHMEAVR LDAEVFMALL RRLLPHVHKH LQQVGVGPLL YLPEWFLCLF ARSLPFPTVL RVWDAFLSEG ARVLFRVGLT LVRLALGTAE QRGACPGLLE TLGALRAIPP AQLQEEAFMS QVHSVVLSER DLQREIKAQL AQLPDSAPGP PPRPQVRLAG AQAIFEAQQL AGVRRGAKPE VPRIVVQPPE EPRPPRRKPQ TRGKTFHGLL TRARGPPIEG PPRPQRGSTS FLDTRF |
| 分子量 | 49.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TBC1D10C蛋白的3篇参考文献示例(注：内容基于领域知识模拟，实际文献可能需要通过数据库进一步检索验证)：
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1. **文献名称**: *TBC1D10C interacts with the mTOR pathway to regulate cancer cell growth and migration*
**作者**: Li Y, et al.
**摘要**: 本研究利用重组TBC1D10C蛋白，揭示其通过结合mTOR复合物调控Rab GTP酶活性，抑制乳腺癌细胞侵袭转移的分子机制。
2. **文献名称**: *Structural characterization of recombinant human TBC1D10C and its interaction with lipid membranes*
**作者**: Smith JM, et al.
**摘要**: 通过重组表达纯化TBC1D10C蛋白，结合冷冻电镜技术解析其三维结构，发现其C端结构域特异结合细胞膜磷脂，提示其在胞内囊泡运输中的调控作用。
3. **文献名称**: *TBC1D10C as a potential biomarker in systemic lupus erythematosus: A functional study using recombinant protein*
**作者**: Wang X, et al.
**摘要**: 通过体外表达重组TBC1D10C，发现其可抑制T细胞过度活化，患者血清中该蛋白表达水平与红斑狼疮疾病活动度负相关，提示其免疫调节功能。
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如需具体文献，建议通过PubMed或Web of Science以关键词“TBC1D10C recombinant”检索，并筛选近年发表的研究。部分文献可能涉及该蛋白的基因功能而非重组表达研究，需注意区分。
TBC1D10C, a member of the TBC (Tre-2/Bub2/Cdc16) domain-containing protein family, functions as a GTPase-activating protein (GAP) primarily targeting Rab GTPases, key regulators of intracellular vesicle trafficking and membrane dynamics. This protein, also known as EPI64C or Carabin, contains a conserved TBC domain critical for Rab GAP activity and a C-terminal region implicated in protein-protein interactions. Structurally, TBC1D10C facilitates the hydrolysis of GTP to GDP on specific Rab proteins, thereby inactivating them to control vesicular transport, receptor recycling, and signal transduction pathways.
Biologically, TBC1D10C is associated with immune regulation, cell proliferation, and cancer progression. Studies link its expression to T-cell receptor signaling and cardiac hypertrophy, where it acts as a dual regulator of Ras/Raf and calcineurin pathways. Notably, TBC1D10C exhibits tumor-suppressive properties in certain cancers (e.g., glioblastoma, leukemia) by inhibiting oncogenic signaling, while paradoxically showing pro-survival roles in others through unclear mechanisms. Dysregulation of TBC1D10C has been observed in multiple cancer types and autoimmune disorders, suggesting its contextual dual roles in disease pathogenesis.
Recombinant TBC1D10C protein is widely utilized in vitro to study Rab-mediated trafficking mechanisms, screen interactors, and explore therapeutic targeting. Its recombinant form enables structural analysis, biochemical assays (e.g., GAP activity), and functional studies in overexpression or knockdown models, advancing insights into intracellular transport and disease-related pathways. Research continues to unravel its tissue-specific functions and potential as a therapeutic target.
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