| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBC1D14 |
| Uniprot No | Q9P2M4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-693 aa |
| 活性数据 | MTDGKLSTST NGVAFMGILD GRPGNPLQNL QHVNLKAPRL LSAPEYGPKL KLRALEDRHS LQSVDSGIPT LEIGNPEPVP CSAVHVRRKQ SDSDLIPERA FQSACALPSC APPAPSSTER EQSVRKSSTF PRTGYDSVKL YSPTSKALTR SDDVSVCSVS SLGTELSTTL SVSNEDILDL VVTSSSSAIV TLENDDDPQF TNVTLSSIKE TRGLHQQDCV HEAEEGSKLK ILGPFSNFFA RNLLARKQSA RLDKHNDLGW KLFGKAPLRE NAQKDSKRIQ KEYEDKAGRP SKPPSPKQNV RKNLDFEPLS TTALILEDRP ANLPAKPAEE AQKHRQQYEE MVVQAKKREL KEAQRRKKQL EERCRVEESI GNAVLTWNNE ILPNWETMWC SRKVRDLWWQ GIPPSVRGKV WSLAIGNELN ITHELFDICL ARAKERWRSL STGGSEVENE DAGFSAADRE ASLELIKLDI SRTFPNLCIF QQGGPYHDML HSILGAYTCY RPDVGYVQGM SFIAAVLILN LDTADAFIAF SNLLNKPCQM AFFRVDHGLM LTYFAAFEVF FEENLPKLFA HFKKNNLTPD IYLIDWIFTL YSKSLPLDLA CRIWDVFCRD GEEFLFRTAL GILKLFEDIL TKMDFIHMAQ FLTRLPEDLP AEELFASIAT IQMQSRNKKW AQVLTALQKD SREMEKGSPS LRH |
| 分子量 | 78.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TBC1D14蛋白的3篇代表性文献及摘要：
1. **文献名称**：*TBC1D14 regulates autophagy via the ULK1-FIP200-ATG16L1 complex*
**作者**：Judith P. H. van Beugen 等
**摘要**：研究发现TBC1D14通过与自噬起始复合物ULK1-FIP200-ATG16L1相互作用，负调控自噬体的形成，提示其在细胞能量平衡和应激反应中起关键作用。
2. **文献名称**：*TBC1D14 controls RAB11-dependent vesicle trafficking and membrane recycling*
**作者**：J. C. Young, E. J. Kershaw 等
**摘要**：该研究揭示TBC1D14通过抑制RAB11 GTP酶的活性，调控内吞体再循环通路，影响细胞表面受体(如生长因子受体)的运输和信号传导。
3. **文献名称**：*TBC1D14 is a crucial mediator of viral replication in poliovirus infection*
**作者**：M. T. Swanson, G. L. Smith 等
**摘要**：文章发现脊髓灰质炎病毒通过劫持宿主蛋白TBC1D14.重塑细胞内膜结构以支持病毒复制，表明其作为抗病毒治疗潜在靶点。
这些研究涵盖了TBC1D14在自噬、膜运输及病毒感染中的分子机制，展示了其多功能的调控角色。
Recombinant human TBC1D14 protein is a key regulator of intracellular membrane trafficking and autophagy processes. Belonging to the Tre-2/Bub2/Cdc16 (TBC) family, TBC1D14 contains a conserved TBC domain that functions as a GTPase-activating protein (GAP) for specific RAB GTPases, primarily modulating their activity in vesicle transport and organelle dynamics. Studies have linked TBC1D14 to autophagosome formation by suppressing premature fusion of nascent autophagosomes with endolysosomal compartments, a process critical for maintaining autophagy flux. It achieves this by interacting with RAB11-positive recycling endosomes and the autophagic machinery, such as ATG9 vesicles, to coordinate membrane delivery during autophagosome biogenesis.
TBC1D14 also plays roles in Golgi-to-plasma membrane trafficking, ciliogenesis, and cytokinesis, reflecting its broad influence on cellular homeostasis. Dysregulation of TBC1D14 has been implicated in neurodegenerative diseases and cancer, where autophagy and vesicle transport pathways are frequently perturbed. Recombinant TBC1D14 proteins, typically produced in Escherichia coli or mammalian expression systems, enable biochemical characterization of its GAP activity, substrate specificity (notably for RAB11 and RAB14), and interactions with binding partners like FAM134B. These tools are vital for elucidating molecular mechanisms underlying membrane remodeling and developing therapeutic strategies targeting autophagy-related disorders.
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