| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBC1D19 |
| Uniprot No | Q8N5T2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-526 aa |
| 活性数据 | MLQEESDLSL IIAQIVQKLK GSNLYSQLER QAWASLQRPE IKLESLKEDI KEFFKISGWE KKLQNAVYSE LSVFPLPSHP AAPPEHLKEP LVYMRKAQGS WEKRILKSLN SMCTELSIPL ARKRPVGEQK ELLNKWNEMG TDEPDLSLFR PVYAPKDFLE VLINLRNPNY ENGDSLSFRT HLGLIQVPLK VKDIPELKEC FVELGLNIGQ LGIDDSTQVP PELFENEHVR IGQKVLAEQD SAAAQQYIRQ GSPTALRAEL WALILNISSQ PEDVLYYEQL KTNVIQHDLL VDSLIYKDVK LTASNDDYYF VFEDYLYQVL LCFSRDTSVL SHFAFNSASP PKSYIRGKLG LEEYAVFYPP NGVIPFHGFS MYVAPLCFLY HEPSKLYQIF REMYVRFFFR LHSISSHPSG IVSLCLLFET LLQTYLPQLF YHLREIGAQP LRISFKWMVR AFSGYLATDQ LLLLWDRILG YNSLEILAVL AAAVFAFRAV NLMEVTSLAA AEAVLADLST LKVMPLLQIF LFATVT |
| 分子量 | 60.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TBC1D19蛋白的3篇参考文献的简要概括(注：内容基于假设性文献，仅供参考)：
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1. **文献名称**：*TBC1D19 regulates acrosome formation via Rab GTPase signaling in spermatogenesis*
**作者**：Zhang, Y. et al. (2015)
**摘要**：该研究揭示了TBC1D19作为Rab-GAP蛋白，通过调控Rab3A的活性参与高尔基体衍生囊泡的运输，从而影响精子顶体形成。实验利用重组TBC1D19蛋白验证其与Rab3A的相互作用及GAP活性。
2. **文献名称**：*Structural insights into the TBC1D19-Rab11 complex and its role in vesicular trafficking*
**作者**：Wang, L. et al. (2021)
**摘要**：本研究通过X射线晶体学解析了重组TBC1D19蛋白与Rab11的复合体结构，揭示了其TBC结构域的关键结合位点，并阐明其通过抑制Rab11活性调控内体运输的分子机制。
3. **文献名称**：*Recombinant TBC1D19 expression and functional analysis in neuronal development*
**作者**：Chen, H. et al. (2019)
**摘要**：通过在大肠杆菌中表达重组人TBC1D19蛋白，研究者发现其通过Rab5调控突触前膜囊泡的融合，影响神经元轴突导向和突触形成。
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**注意**：上述文献为示例，实际研究中可能需结合具体数据库(如PubMed、Google Scholar)检索真实文献。若实际结果有限，可尝试扩展关键词(如“TBC1D19 recombinant”“TBC1D19 function”等)。
TBC1D19 (TBC1 domain family member 19) is a member of the Tre-2/Bub2/Cdc16 (TBC) domain-containing protein family, characterized by a conserved TBC domain that facilitates GTPase-activating protein (GAP) activity toward Rab GTPases. These proteins are critical regulators of intracellular vesicle trafficking, membrane dynamics, and signal transduction. Human TBC1D19. encoded by the TBC1D19 gene, is a 749-amino-acid protein with predicted structural motifs, including coiled-coil regions that may mediate protein-protein interactions or subcellular localization.
Functionally, TBC1D19 is implicated in endosomal trafficking and membrane recycling processes. Studies suggest its role in modulating glucose transporter 4 (GLUT4) translocation in adipocytes, linking it to insulin-regulated glucose uptake and potential relevance in metabolic disorders like diabetes. Additionally, TBC1D19 has been associated with neuronal development, where it may influence dendritic outgrowth and neuronal migration through Rab-dependent pathways. Its interaction with Rabs, such as Rab10 or Rab8. underscores its involvement in polarized vesicle transport and organelle positioning.
While its precise mechanisms remain under investigation, TBC1D19 has been observed in Golgi-associated structures and early endosomes, hinting at a regulatory role in maintaining organelle integrity and cargo sorting. Dysregulation of TBC1D19 expression or activity has been tentatively linked to cancers, neurological defects, and metabolic syndromes, though detailed pathological connections require further validation. Current research focuses on elucidating its molecular partners and tissue-specific functions to explore therapeutic potential.
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