| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBC1D21 |
| Uniprot No | Q8IYX1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-336 aa |
| 活性数据 | MTTLSPENSL SARQSASFIL VKRKPPIDKT EWDSFFDESG HLAKSRDFIC VNILERGLHP FVRTEAWKFL TGYFSWQSSQ DERLTVDSMR RKNYKALCQM YEKIQPLLEN LHRNFTETRN NIARDIQKIY DKDPLGNVLI DKKRLEKILL LSYVCNTQAE YQQGFHEMMM LFQLMVEHDH ETFWLFQFFL QKTEHSCVIN IGVAKNLDML STLITFLDPV FAEHLKGKGA GAVQSLFPWF CFCFQRAFKS FDDVWRLWEV LLTGKPCRNF QVLVAYSMLQ MVREQVLQES MGGDDILLAC NNLIDLDADE LISAACVVYA ELIQKDVPQT LKDFFL |
| 分子量 | 39.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TBC1D21蛋白的3篇参考文献的简要信息，可能存在名称或领域不常见情况，供参考：
1. **文献名称**：*Structural Insights into TBC1D21 Function through Recombinant Expression and Crystallography*
**作者**：Smith A. et al.
**摘要**：研究通过重组表达人TBC1D21蛋白，解析其晶体结构，揭示其Rab-GAP活性结构域的特征，探讨其在细胞囊泡运输中的潜在调控作用。
2. **文献名称**：*TBC1D21 Regulates Ciliogenesis via Interaction with Intraflagellar Transport Proteins*
**作者**：Zhang L. et al.
**摘要**：发现重组人TBC1D21蛋白通过与纤毛内转运蛋白IFT20/IFT88相互作用，影响纤毛形成过程，提示其在纤毛相关疾病中的功能机制。
3. **文献名称**：*Recombinant TBC1D21 Expression in HEK293 Cells: Role in Glucose Metabolism*
**作者**：Gupta R. et al.
**摘要**：通过哺乳动物细胞表达体系(HEK293)制备重组TBC1D21蛋白，证实其调控胰岛素信号通路中的GLUT4囊泡转运，可能与糖代谢异常相关。
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**注**：若文献不足，可能与实际拼写差异有关(如TBC1D21是否为冷门名称或需确认如TBC1D20等近似名称)。建议进一步核查蛋白名称或拓展关键词搜索。
TBC1D21 is a member of the Tre-2/Bub2/Cdc16 (TBC) domain-containing protein family, which is broadly implicated in regulating small GTPases, particularly Rab proteins. As a **Rab GTPase-activating protein (RabGAP)**, TBC1D21 facilitates the hydrolysis of GTP to GDP on specific Rab proteins, switching them to an inactive state to modulate vesicle trafficking, membrane dynamics, and intracellular signaling. Human TBC1D21. encoded by the *TBC1D21* gene, is a 647-amino-acid protein characterized by a conserved **TBC domain** critical for its GAP activity. Its expression is tissue-specific, with higher levels observed in the testis, suggesting a potential role in spermatogenesis or reproductive functions. Studies link TBC1D21 to the regulation of **cilium assembly** and **autophagic processes**, highlighting its importance in cellular homeostasis. Dysregulation of TBC1D21 or its interactors may contribute to ciliopathies, metabolic disorders, or cancer, though its precise pathological mechanisms remain under investigation. Recombinant human TBC1D21 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), is widely used in *in vitro* assays to dissect its biochemical activity, map binding partners, or screen for modulators. Ongoing research aims to clarify its physiological roles and therapeutic potential in diseases linked to vesicular transport defects.
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