| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBL1XR1 |
| Uniprot No | Q9BZK7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-514 aa |
| 活性数据 | SISSDEVNF LVYRYLQESG FSHSAFTFGI ESHISQSNIN GALVPPAALI SIIQKGLQYV EAEVSINEDG TLFDGRPIES LSLIDAVMPD VVQTRQQAYR DKLAQQQAAA AAAAAAAASQ QGSAKNGENT ANGEENGAHT IANNHTDMME VDGDVEIPPN KAVVLRGHES EVFICAWNPV SDLLASGSGD STARIWNLSE NSTSGSTQLV LRHCIREGGQ DVPSNKDVTS LDWNSEGTLL ATGSYDGFAR IWTKDGNLAS TLGQHKGPIF ALKWNKKGNF ILSAGVDKTT IIWDAHTGEA KQQFPFHSAP ALDVDWQSNN TFASCSTDMC IHVCKLGQDR PIKTFQGHTN EVNAIKWDPT GNLLASCSDD MTLKIWSMKQ DNCVHDLQAH NKEIYTIKWS PTGPGTNNPN ANLMLASASF DSTVRLWDVD RGICIHTLTK HQEPVYSVAF SPDGRYLASG SFDKCVHIWN TQTGALVHSY RGTGGIFEVC WNAAGDKVGA SASDGSVCVL DLRK |
| 分子量 | 55.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TBL1XR1蛋白的3篇参考文献示例(注：文献信息为虚构，仅作格式参考)：
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1. **文献名称**：*Structural insights into TBL1XR1-mediated transcriptional regulation by recombinant protein analysis*
**作者**：Li et al.
**摘要**：通过重组表达和纯化人TBL1XR1蛋白，结合X射线晶体学解析其三维结构，揭示其与核受体复合物的相互作用界面，阐明其在Wnt/β-catenin信号通路中的调控机制。
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2. **文献名称**：*TBL1XR1 mutations disrupt cerebellar development via impaired protein stability*
**作者**：Wang et al.
**摘要**：研究显示，TBL1XR1基因突变导致重组蛋白稳定性降低，破坏其与小脑发育相关转录因子(如TCF4)的结合，引发共济失调等神经发育障碍。
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3. **文献名称**：*Recombinant TBL1XR1 promotes cancer metastasis through enhancing EGFR signaling*
**作者**：Chen et al.
**摘要**：利用重组TBL1XR1蛋白进行体外实验，发现其通过增强EGFR信号转导，促进乳腺癌细胞侵袭和转移，为靶向治疗提供新方向。
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注：实际文献建议通过 **PubMed** 或 **Google Scholar** 检索关键词“TBL1XR1 recombinant”、“TBL1XR1 structure/function”获取。若需真实文献，可提供具体数据库检索结果。
**Background of Recombinant Human TBL1XR1 Protein**
TBL1XR1 (Transducin beta-like 1 X-linked receptor 1) is a core component of the nuclear receptor corepressor (N-CoR) and silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) complexes, which play critical roles in transcriptional regulation. This protein contains WD40 repeat domains that mediate protein-protein interactions, facilitating the recruitment of histone deacetylases (HDACs) to repress target gene expression. TBL1XR1 also participates in switching corepressor complexes to coactivator complexes during ligand-dependent transcriptional activation, linking nuclear receptors and signaling pathways like Wnt/β-catenin and NF-κB.
Recombinant human TBL1XR1 protein is produced using expression systems (e.g., *E. coli* or mammalian cells) to enable functional and structural studies. Its applications include investigating molecular mechanisms of transcriptional regulation, protein interaction networks, and disease-associated mutations. Dysregulation of TBL1XR1 is linked to cancers, neurodevelopmental disorders (e.g., autism, intellectual disability), and Coffin-Siris syndrome. Mutations or overexpression of TBL1XR1 may disrupt chromatin remodeling, leading to aberrant cell proliferation or differentiation.
Studying recombinant TBL1XR1 aids in elucidating its role in gene silencing/activation, disease pathways, and therapeutic targeting. Its WD40 domains and phosphorylation sites are of particular interest for understanding interaction specificity and regulatory modifications.
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